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1js4

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[[Image:1js4.gif|left|200px]]<br /><applet load="1js4" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1js4.gif|left|200px]]
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caption="1js4, resolution 2.0&Aring;" />
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'''ENDO/EXOCELLULASE:CELLOBIOSE FROM THERMOMONOSPORA'''<br />
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{{Structure
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|PDB= 1js4 |SIZE=350|CAPTION= <scene name='initialview01'>1js4</scene>, resolution 2.0&Aring;
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|SITE= <scene name='pdbsite=AA1:Active+Site'>AA1</scene>, <scene name='pdbsite=AB1:Active+Site'>AB1</scene>, <scene name='pdbsite=CA1:Ca+Binding+Site'>CA1</scene>, <scene name='pdbsite=CA2:Ca+Binding+Site'>CA2</scene>, <scene name='pdbsite=CA3:Ca+Binding+Site'>CA3</scene> and <scene name='pdbsite=CA4:Ca+Binding+Site'>CA4</scene>
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|LIGAND= <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene> and <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]
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|GENE= BAMH1-PST1 FRAGMENT OF T. FUSC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2021 Thermobifida fusca])
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}}
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'''ENDO/EXOCELLULASE:CELLOBIOSE FROM THERMOMONOSPORA'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1JS4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca] with <scene name='pdbligand=GLC:'>GLC</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Known structural/functional Sites: <scene name='pdbsite=AA1:Active+Site'>AA1</scene>, <scene name='pdbsite=AB1:Active+Site'>AB1</scene>, <scene name='pdbsite=CA1:Ca+Binding+Site'>CA1</scene>, <scene name='pdbsite=CA2:Ca+Binding+Site'>CA2</scene>, <scene name='pdbsite=CA3:Ca+Binding+Site'>CA3</scene> and <scene name='pdbsite=CA4:Ca+Binding+Site'>CA4</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JS4 OCA].
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1JS4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JS4 OCA].
==Reference==
==Reference==
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Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca., Sakon J, Irwin D, Wilson DB, Karplus PA, Nat Struct Biol. 1997 Oct;4(10):810-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9334746 9334746]
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Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca., Sakon J, Irwin D, Wilson DB, Karplus PA, Nat Struct Biol. 1997 Oct;4(10):810-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9334746 9334746]
[[Category: Cellulase]]
[[Category: Cellulase]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: glycosyl hydrolase]]
[[Category: glycosyl hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:26:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:07:43 2008''

Revision as of 10:07, 20 March 2008

Image:1js4.gif


PDB ID 1js4

Drag the structure with the mouse to rotate
, resolution 2.0Å
Sites: , , , , and
Ligands: and
Gene: BAMH1-PST1 FRAGMENT OF T. FUSC (Thermobifida fusca)
Activity: Cellulase, with EC number 3.2.1.4
Coordinates: save as pdb, mmCIF, xml



ENDO/EXOCELLULASE:CELLOBIOSE FROM THERMOMONOSPORA


Overview

Cellulase E4 from Thermomonospora fusca is unusual in that it has characteristics of both exo- and endo-cellulases. Here we report the crystal structure of a 68K M(r) fragment of E4 (E4-68) at 1.9 A resolution. E4-68 contains both a family 9 catalytic domain, exhibiting an (alpha/alpha)6 barrel fold, and a family III cellulose binding domain, having an antiparallel beta-sandwich fold. While neither of these folds is novel, E4-68 provides the first cellulase structure having interacting catalytic and cellulose binding domains. The complexes of E4-68 with cellopentaose, cellotriose and cellobiose reveal conformational changes associated with ligand binding and allow us to propose a catalytic mechanism for family 9 enzymes. We also provide evidence that E4 has two novel characteristics: first it combines exo- and endo-activities and second, when it functions as an exo-cellulase, it cleaves off cellotetraose units.

About this Structure

1JS4 is a Single protein structure of sequence from Thermobifida fusca. Full crystallographic information is available from OCA.

Reference

Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca., Sakon J, Irwin D, Wilson DB, Karplus PA, Nat Struct Biol. 1997 Oct;4(10):810-8. PMID:9334746

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