This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1jxg
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1jxg.gif|left|200px]] | + | [[Image:1jxg.gif|left|200px]] |
| - | + | ||
| - | '''The 1.6 A Resolution Crystal Structure of a Mutant Poplar Plastocyanin Bearing a 21-25 Engeneered Disulfide Bridge''' | + | {{Structure |
| + | |PDB= 1jxg |SIZE=350|CAPTION= <scene name='initialview01'>1jxg</scene>, resolution 1.6Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''The 1.6 A Resolution Crystal Structure of a Mutant Poplar Plastocyanin Bearing a 21-25 Engeneered Disulfide Bridge''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1JXG is a [ | + | 1JXG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Populus_nigra Populus nigra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JXG OCA]. |
==Reference== | ==Reference== | ||
| - | The 1.6 A resolution crystal structure of a mutant plastocyanin bearing a 21-25 engineered disulfide bridge., Milani M, Andolfi L, Cannistraro S, Verbeet MP, Bolognesi M, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1735-8. Epub 2001, Oct 25. PMID:[http:// | + | The 1.6 A resolution crystal structure of a mutant plastocyanin bearing a 21-25 engineered disulfide bridge., Milani M, Andolfi L, Cannistraro S, Verbeet MP, Bolognesi M, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1735-8. Epub 2001, Oct 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11679761 11679761] |
[[Category: Populus nigra]] | [[Category: Populus nigra]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 22: | Line 31: | ||
[[Category: beta barrel]] | [[Category: beta barrel]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:09:46 2008'' |
Revision as of 10:09, 20 March 2008
| |||||||
| , resolution 1.6Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The 1.6 A Resolution Crystal Structure of a Mutant Poplar Plastocyanin Bearing a 21-25 Engeneered Disulfide Bridge
Overview
Plastocyanin is an electron-transfer protein which has been largely used for biophysical studies as well as for protein-engineering experiments. A surface disulfide bridge has been engineered in poplar plastocyanin to allow protein chemisorption on gold substrates. The mutated plastocyanin crystal structure has been studied at 1.6 A resolution (R factor = 0.145, R(free) = 0.205) to characterize the effects of the engineered disulfide on the overall protein structure and on the Cu-coordination sphere in view of biophysical applications. The new orthorhombic crystal form isolated for the mutated plastocyanin displays two protein molecules per asymmetric unit.
About this Structure
1JXG is a Single protein structure of sequence from Populus nigra. Full crystallographic information is available from OCA.
Reference
The 1.6 A resolution crystal structure of a mutant plastocyanin bearing a 21-25 engineered disulfide bridge., Milani M, Andolfi L, Cannistraro S, Verbeet MP, Bolognesi M, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1735-8. Epub 2001, Oct 25. PMID:11679761
Page seeded by OCA on Thu Mar 20 12:09:46 2008
Categories: Populus nigra | Single protein | Andolfi, L. | Bolognesi, M. | Cannistraro, S. | Milani, M. | Verbeet, M P. | CU1 | SO4 | Beta barrel
