1jxg

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[[Image:1jxg.gif|left|200px]]<br /><applet load="1jxg" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1jxg.gif|left|200px]]
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caption="1jxg, resolution 1.6&Aring;" />
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'''The 1.6 A Resolution Crystal Structure of a Mutant Poplar Plastocyanin Bearing a 21-25 Engeneered Disulfide Bridge'''<br />
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{{Structure
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|PDB= 1jxg |SIZE=350|CAPTION= <scene name='initialview01'>1jxg</scene>, resolution 1.6&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''The 1.6 A Resolution Crystal Structure of a Mutant Poplar Plastocyanin Bearing a 21-25 Engeneered Disulfide Bridge'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1JXG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Populus_nigra Populus nigra] with <scene name='pdbligand=CU1:'>CU1</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JXG OCA].
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1JXG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Populus_nigra Populus nigra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JXG OCA].
==Reference==
==Reference==
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The 1.6 A resolution crystal structure of a mutant plastocyanin bearing a 21-25 engineered disulfide bridge., Milani M, Andolfi L, Cannistraro S, Verbeet MP, Bolognesi M, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1735-8. Epub 2001, Oct 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11679761 11679761]
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The 1.6 A resolution crystal structure of a mutant plastocyanin bearing a 21-25 engineered disulfide bridge., Milani M, Andolfi L, Cannistraro S, Verbeet MP, Bolognesi M, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1735-8. Epub 2001, Oct 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11679761 11679761]
[[Category: Populus nigra]]
[[Category: Populus nigra]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: beta barrel]]
[[Category: beta barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:27:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:09:46 2008''

Revision as of 10:09, 20 March 2008

Image:1jxg.gif


PDB ID 1jxg

Drag the structure with the mouse to rotate
, resolution 1.6Å
Ligands: and
Coordinates: save as pdb, mmCIF, xml



The 1.6 A Resolution Crystal Structure of a Mutant Poplar Plastocyanin Bearing a 21-25 Engeneered Disulfide Bridge


Overview

Plastocyanin is an electron-transfer protein which has been largely used for biophysical studies as well as for protein-engineering experiments. A surface disulfide bridge has been engineered in poplar plastocyanin to allow protein chemisorption on gold substrates. The mutated plastocyanin crystal structure has been studied at 1.6 A resolution (R factor = 0.145, R(free) = 0.205) to characterize the effects of the engineered disulfide on the overall protein structure and on the Cu-coordination sphere in view of biophysical applications. The new orthorhombic crystal form isolated for the mutated plastocyanin displays two protein molecules per asymmetric unit.

About this Structure

1JXG is a Single protein structure of sequence from Populus nigra. Full crystallographic information is available from OCA.

Reference

The 1.6 A resolution crystal structure of a mutant plastocyanin bearing a 21-25 engineered disulfide bridge., Milani M, Andolfi L, Cannistraro S, Verbeet MP, Bolognesi M, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1735-8. Epub 2001, Oct 25. PMID:11679761

Page seeded by OCA on Thu Mar 20 12:09:46 2008

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