1jy6
From Proteopedia
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| - | [[Image:1jy6.gif|left|200px]] | + | [[Image:1jy6.gif|left|200px]] |
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| - | '''B4DIMERA: A DE NOVO DESIGNED FOUR-STRANDED BETA-SHEET ASSEMBLED USING A DISULFIDE BOND''' | + | {{Structure |
| + | |PDB= 1jy6 |SIZE=350|CAPTION= <scene name='initialview01'>1jy6</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''B4DIMERA: A DE NOVO DESIGNED FOUR-STRANDED BETA-SHEET ASSEMBLED USING A DISULFIDE BOND''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1JY6 is a [ | + | 1JY6 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JY6 OCA]. |
==Reference== | ==Reference== | ||
| - | Design and construction of an open multistranded beta-sheet polypeptide stabilized by a disulfide bridge., Venkatraman J, Nagana Gowda GA, Balaram P, J Am Chem Soc. 2002 May 8;124(18):4987-94. PMID:[http:// | + | Design and construction of an open multistranded beta-sheet polypeptide stabilized by a disulfide bridge., Venkatraman J, Nagana Gowda GA, Balaram P, J Am Chem Soc. 2002 May 8;124(18):4987-94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11982362 11982362] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Balaram, P.]] | [[Category: Balaram, P.]] | ||
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[[Category: four-stranded beta-sheet]] | [[Category: four-stranded beta-sheet]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:10:02 2008'' |
Revision as of 10:10, 20 March 2008
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B4DIMERA: A DE NOVO DESIGNED FOUR-STRANDED BETA-SHEET ASSEMBLED USING A DISULFIDE BOND
Overview
The design and characterization of an open eight-stranded beta-sheet in a synthetic, 2-fold symmetric 70-residue peptide is described. The design strategy involves the generation of a 35-residue four-stranded beta-sheet peptide in which successive hairpins are nucleated by appropriately positioned (D)Pro-Xxx sequences. Oxidative dimerization using a single Cys residue positioned at the center of the C-terminal strand results in a disulfide-bridged eight-stranded structure. Nuclear Overhauser effects firmly establish an eight-stranded beta-sheet in methanol. In water, the outer strands are frayed, but a well-defined four-stranded beta-sheet stabilized by a disulfide bridge and a hydrophobic cluster is determined from NMR data. Comparison of the precursor peptide with the disulfide-bridged dimer reveals considerable enhancement of beta-sheet content in the latter, suggesting that the disulfide cross-link is an effective strategy for the stabilization of beta-sheets.
About this Structure
1JY6 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Design and construction of an open multistranded beta-sheet polypeptide stabilized by a disulfide bridge., Venkatraman J, Nagana Gowda GA, Balaram P, J Am Chem Soc. 2002 May 8;124(18):4987-94. PMID:11982362
Page seeded by OCA on Thu Mar 20 12:10:02 2008
