1k07
From Proteopedia
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| - | [[Image:1k07.gif|left|200px]] | + | [[Image:1k07.gif|left|200px]] |
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| - | '''Native FEZ-1 metallo-beta-lactamase from Legionella gormanii''' | + | {{Structure |
| + | |PDB= 1k07 |SIZE=350|CAPTION= <scene name='initialview01'>1k07</scene>, resolution 1.65Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] | ||
| + | |GENE= blaFEZ-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=464 Fluoribacter gormanii]) | ||
| + | }} | ||
| + | |||
| + | '''Native FEZ-1 metallo-beta-lactamase from Legionella gormanii''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1K07 is a [ | + | 1K07 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Fluoribacter_gormanii Fluoribacter gormanii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K07 OCA]. |
==Reference== | ==Reference== | ||
| - | Three-dimensional structure of FEZ-1, a monomeric subclass B3 metallo-beta-lactamase from Fluoribacter gormanii, in native form and in complex with D-captopril., Garcia-Saez I, Mercuri PS, Papamicael C, Kahn R, Frere JM, Galleni M, Rossolini GM, Dideberg O, J Mol Biol. 2003 Jan 24;325(4):651-60. PMID:[http:// | + | Three-dimensional structure of FEZ-1, a monomeric subclass B3 metallo-beta-lactamase from Fluoribacter gormanii, in native form and in complex with D-captopril., Garcia-Saez I, Mercuri PS, Papamicael C, Kahn R, Frere JM, Galleni M, Rossolini GM, Dideberg O, J Mol Biol. 2003 Jan 24;325(4):651-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12507470 12507470] |
[[Category: Beta-lactamase]] | [[Category: Beta-lactamase]] | ||
[[Category: Fluoribacter gormanii]] | [[Category: Fluoribacter gormanii]] | ||
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[[Category: monomer with alpha-beta/beta-alpha fold. two monomers per assymmetric unit.]] | [[Category: monomer with alpha-beta/beta-alpha fold. two monomers per assymmetric unit.]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:10:46 2008'' |
Revision as of 10:10, 20 March 2008
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| , resolution 1.65Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , and | ||||||
| Gene: | blaFEZ-1 (Fluoribacter gormanii) | ||||||
| Activity: | Beta-lactamase, with EC number 3.5.2.6 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Native FEZ-1 metallo-beta-lactamase from Legionella gormanii
Overview
The beta-lactamases are involved in bacterial resistance to penicillin and related compounds. Members of the metallo-enzyme class are now found in many pathogenic bacteria and are thus becoming of major clinical importance. The structures of the Zn-beta-lactamase from Fluoribacter gormanii (FEZ-1) in the native and in the complex form are reported here. FEZ-1 is a monomeric enzyme, which possesses two zinc-binding sites. These structures are discussed in comparison with those of the tetrameric L1 enzyme produced by Stenotrophomonas maltophilia. From this analysis, amino acids involved in the oligomerization of L1 are clearly identified. Despite the similarity in fold, the active site of FEZ-1 was found to be significantly different. Two residues, which were previously implicated in function, are not present in L1 or in FEZ-1. The broad-spectrum substrate profile of Zn-beta-lactamases arises from the rather wide active-site cleft, where various beta-lactam compounds can be accommodated.
About this Structure
1K07 is a Single protein structure of sequence from Fluoribacter gormanii. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of FEZ-1, a monomeric subclass B3 metallo-beta-lactamase from Fluoribacter gormanii, in native form and in complex with D-captopril., Garcia-Saez I, Mercuri PS, Papamicael C, Kahn R, Frere JM, Galleni M, Rossolini GM, Dideberg O, J Mol Biol. 2003 Jan 24;325(4):651-60. PMID:12507470
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