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1k19
From Proteopedia
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| - | [[Image:1k19.jpg|left|200px]] | + | [[Image:1k19.jpg|left|200px]] |
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| - | '''NMR Solution Structure of the Chemosensory Protein CSP2 from Moth Mamestra brassicae''' | + | {{Structure |
| + | |PDB= 1k19 |SIZE=350|CAPTION= <scene name='initialview01'>1k19</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''NMR Solution Structure of the Chemosensory Protein CSP2 from Moth Mamestra brassicae''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1K19 is a [ | + | 1K19 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mamestra_brassicae Mamestra brassicae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K19 OCA]. |
==Reference== | ==Reference== | ||
| - | Solution structure of a chemosensory protein from the moth Mamestra brassicae., Mosbah A, Campanacci V, Lartigue A, Tegoni M, Cambillau C, Darbon H, Biochem J. 2003 Jan 1;369(Pt 1):39-44. PMID:[http:// | + | Solution structure of a chemosensory protein from the moth Mamestra brassicae., Mosbah A, Campanacci V, Lartigue A, Tegoni M, Cambillau C, Darbon H, Biochem J. 2003 Jan 1;369(Pt 1):39-44. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12217077 12217077] |
[[Category: Mamestra brassicae]] | [[Category: Mamestra brassicae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: pheromone]] | [[Category: pheromone]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:11:09 2008'' |
Revision as of 10:11, 20 March 2008
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NMR Solution Structure of the Chemosensory Protein CSP2 from Moth Mamestra brassicae
Overview
Chemosensory proteins (CSPs) are believed to be involved in chemical communication and perception. A number of such proteins, of molecular mass approximately 13 kDa, have been isolated from different sensory organs of a wide range of insect species. Several CSPs have been identified in the antennae and proboscis of the moth Mamestra brassicae. CSPMbraA6, a 112-amino-acid antennal protein, has been expressed in a soluble form in large quantities in the Escherichi coli periplasm. NMR structure determination of CSPMbraA6 has been performed with 1H- and 15N-labelled samples. The calculated structures present an average root mean square deviation about the mean structure of 0.63 A for backbone atoms and 1.27 A for all non-hydrogen atoms except the 12 N-terminal residues. The protein is well folded from residue 12 to residue 110, and consists of a non-bundle alpha-helical structure with six helices connected by alpha alpha loops. It has a globular shape, with overall dimensions of 32 A x 28 A x 24 A. A channel is visible in the hydrophobic core, with dimensions of 3 A x 9 A x 21 A. In some of the 20 solution structures calculated, this channel is closed either by Trp-94 at one end or by Tyr-26 at the other end; in some other solutions, this channel is closed at both ends. Binding experiments with 12-bromododecanol indicate that the CSPMbraA6 structure is modified upon ligand binding.
About this Structure
1K19 is a Single protein structure of sequence from Mamestra brassicae. Full crystallographic information is available from OCA.
Reference
Solution structure of a chemosensory protein from the moth Mamestra brassicae., Mosbah A, Campanacci V, Lartigue A, Tegoni M, Cambillau C, Darbon H, Biochem J. 2003 Jan 1;369(Pt 1):39-44. PMID:12217077
Page seeded by OCA on Thu Mar 20 12:11:09 2008
