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1k1z
From Proteopedia
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| - | [[Image:1k1z.gif|left|200px]] | + | [[Image:1k1z.gif|left|200px]] |
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| - | '''Solution structure of N-terminal SH3 domain mutant(P33G) of murine Vav''' | + | {{Structure |
| + | |PDB= 1k1z |SIZE=350|CAPTION= <scene name='initialview01'>1k1z</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Solution structure of N-terminal SH3 domain mutant(P33G) of murine Vav''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1K1Z is a [ | + | 1K1Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K1Z OCA]. |
==Reference== | ==Reference== | ||
| - | Solution structure of N-terminal SH3 domain of Vav and the recognition site for Grb2 C-terminal SH3 domain., Ogura K, Nagata K, Horiuchi M, Ebisui E, Hasuda T, Yuzawa S, Nishida M, Hatanaka H, Inagaki F, J Biomol NMR. 2002 Jan;22(1):37-46. PMID:[http:// | + | Solution structure of N-terminal SH3 domain of Vav and the recognition site for Grb2 C-terminal SH3 domain., Ogura K, Nagata K, Horiuchi M, Ebisui E, Hasuda T, Yuzawa S, Nishida M, Hatanaka H, Inagaki F, J Biomol NMR. 2002 Jan;22(1):37-46. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11885979 11885979] |
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: sh3]] | [[Category: sh3]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:11:25 2008'' |
Revision as of 10:11, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of N-terminal SH3 domain mutant(P33G) of murine Vav
Overview
The three-dimensional structure of the N-terminal SH3 domain (residues 583-660) of murine Vav, which contains a tetra-proline sequence (Pro 607-Pro 610), was determined by NMR. The solution structure of the SH3 domain shows a typical SH3 fold, but it exists in two conformations due to cis-trans isomerization at the Gly614-Pro615 bond. The NMR structure of the P615G mutant, where Pro615 is replaced by glycine, reveals that the tetra-proline region is inserted into the RT-loop and binds to its own SH3 structure. The C-terminal SH3 domain of Grb2 specifically binds to the trans form of the N-terminal SH3 domain of Vav. The surface of Vav N-terminal SH3 which binds to Grb2 C-terminal SH3 was elucidated by chemical shift mapping experiments using NMR. The surface does not involve the tetra-proline region but involves the region comprising the n-src loop, the N-terminal and the C-terminal regions. This surface is located opposite to the tetra-proline containing region, consistent with that of our previous mutagenesis studies.
About this Structure
1K1Z is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Solution structure of N-terminal SH3 domain of Vav and the recognition site for Grb2 C-terminal SH3 domain., Ogura K, Nagata K, Horiuchi M, Ebisui E, Hasuda T, Yuzawa S, Nishida M, Hatanaka H, Inagaki F, J Biomol NMR. 2002 Jan;22(1):37-46. PMID:11885979
Page seeded by OCA on Thu Mar 20 12:11:25 2008
Categories: Mus musculus | Single protein | Ebisui, E. | Hasuda, T. | Hatanaka, H. | Horiuchi, M. | Inagaki, F. | Nagata, K. | Nishida, M. | Ogura, K. | Yuzawa, S. | Proto-oncogene | Sh3
