1k5k

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[[Image:1k5k.gif|left|200px]]<br /><applet load="1k5k" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1k5k.gif|left|200px]]
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caption="1k5k" />
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'''Homonuclear 1H Nuclear Magnetic Resonance Assignment and Structural Characterization of HIV-1 Tat Mal Protein'''<br />
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{{Structure
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|PDB= 1k5k |SIZE=350|CAPTION= <scene name='initialview01'>1k5k</scene>
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE=
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}}
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'''Homonuclear 1H Nuclear Magnetic Resonance Assignment and Structural Characterization of HIV-1 Tat Mal Protein'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1K5K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K5K OCA].
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1K5K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K5K OCA].
==Reference==
==Reference==
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Homonuclear (1)H-NMR assignment and structural characterization of human immunodeficiency virus type 1 Tat Mal protein., Gregoire C, Peloponese JM Jr, Esquieu D, Opi S, Campbell G, Solomiac M, Lebrun E, Lebreton J, Loret EP, Biopolymers. 2001;62(6):324-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11857271 11857271]
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Homonuclear (1)H-NMR assignment and structural characterization of human immunodeficiency virus type 1 Tat Mal protein., Gregoire C, Peloponese JM Jr, Esquieu D, Opi S, Campbell G, Solomiac M, Lebrun E, Lebreton J, Loret EP, Biopolymers. 2001;62(6):324-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11857271 11857271]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Campbell, G.]]
[[Category: Campbell, G.]]
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[[Category: tat]]
[[Category: tat]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:30:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:12:54 2008''

Revision as of 10:12, 20 March 2008

Image:1k5k.gif


PDB ID 1k5k

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Coordinates: save as pdb, mmCIF, xml



Homonuclear 1H Nuclear Magnetic Resonance Assignment and Structural Characterization of HIV-1 Tat Mal Protein


Overview

The transacting transcriptional activator (Tat) is a viral protein essential for activation of the human immunodeficiency virus (HIV) genes, and it plays an important role in HIV induced immunodeficiency. We report the NMR structural characterization of the active Tat Mal variant that belongs to a highly virulent D-subtype HIV type-1 (HIV-1) strain (Mal) found mainly in Africa. A full Tat Mal protein (87 residues) is synthesized. This synthetic protein is active in a transactivation assay with HeLa cells infected with the HIV long terminal repeated noncoding sequences of the HIV-1 provirus (LTR) lac Z gene. Homonuclear (1)H-NMR spectra allows the sequential assignment of the Tat Mal spin systems. Simulating annealing generates 20 conformers with similar folding. The geometry of the mean structure is optimized with energy minimization to obtain a final structure. As the European variant (Tat Bru) the N-terminal region of Tat Mal constitutes the core, and there is a hydrophobic pocket composed of the conserved Trp 11 interacting with several aromatic residues. The two functional regions of Tat (basic and the cysteine-rich regions) are well exposed to the solvent. A short alpha-helix is observed in region V adjacent to the basic region. This alpha helix induces local structural variations compared to the NMR structure of Tat Bru, and it brings the cysteine-rich and basic regions closer. This study suggests that similar folding exists among Tat variants.

About this Structure

1K5K is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.

Reference

Homonuclear (1)H-NMR assignment and structural characterization of human immunodeficiency virus type 1 Tat Mal protein., Gregoire C, Peloponese JM Jr, Esquieu D, Opi S, Campbell G, Solomiac M, Lebrun E, Lebreton J, Loret EP, Biopolymers. 2001;62(6):324-35. PMID:11857271

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