1k86

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[[Image:1k86.gif|left|200px]]<br /><applet load="1k86" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1k86.gif|left|200px]]
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caption="1k86, resolution 2.6&Aring;" />
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'''Crystal structure of caspase-7'''<br />
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{{Structure
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|PDB= 1k86 |SIZE=350|CAPTION= <scene name='initialview01'>1k86</scene>, resolution 2.6&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE= caspase-7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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}}
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'''Crystal structure of caspase-7'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1K86 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K86 OCA].
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1K86 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K86 OCA].
==Reference==
==Reference==
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Crystal structure of a procaspase-7 zymogen: mechanisms of activation and substrate binding., Chai J, Wu Q, Shiozaki E, Srinivasula SM, Alnemri ES, Shi Y, Cell. 2001 Nov 2;107(3):399-407. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11701129 11701129]
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Crystal structure of a procaspase-7 zymogen: mechanisms of activation and substrate binding., Chai J, Wu Q, Shiozaki E, Srinivasula SM, Alnemri ES, Shi Y, Cell. 2001 Nov 2;107(3):399-407. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11701129 11701129]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: zymogen]]
[[Category: zymogen]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:31:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:13:50 2008''

Revision as of 10:13, 20 March 2008

Image:1k86.gif


PDB ID 1k86

Drag the structure with the mouse to rotate
, resolution 2.6Å
Gene: caspase-7 (Homo sapiens)
Coordinates: save as pdb, mmCIF, xml



Crystal structure of caspase-7


Overview

Apoptosis is primarily executed by active caspases, which are derived from the inactive procaspase zymogens through proteolytic cleavage. Here we report the crystal structures of a caspase zymogen, procaspase-7, and an active caspase-7 without any bound inhibitors. Compared to the inhibitor-bound caspase-7, procaspase-7 zymogen exhibits significant structural differences surrounding the catalytic cleft, which precludes the formation of a productive conformation. Proteolytic cleavage between the large and small subunits allows rearrangement of essential loops in the active site, priming active caspase-7 for inhibitor/substrate binding. Strikingly, binding by inhibitors causes a 180 degrees flipping of the N terminus in the small subunit, which interacts with and stabilizes the catalytic cleft. These analyses reveal the structural mechanisms of caspase activation and demonstrate that the inhibitor/substrate binding is a process of induced fit.

About this Structure

1K86 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of a procaspase-7 zymogen: mechanisms of activation and substrate binding., Chai J, Wu Q, Shiozaki E, Srinivasula SM, Alnemri ES, Shi Y, Cell. 2001 Nov 2;107(3):399-407. PMID:11701129

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