1k8w
From Proteopedia
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| - | [[Image:1k8w.gif|left|200px]] | + | [[Image:1k8w.gif|left|200px]] |
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| - | '''Crystal structure of the E. coli pseudouridine synthase TruB bound to a T stem-loop RNA''' | + | {{Structure |
| + | |PDB= 1k8w |SIZE=350|CAPTION= <scene name='initialview01'>1k8w</scene>, resolution 1.85Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Pseudouridylate_synthase Pseudouridylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.70 4.2.1.70] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the E. coli pseudouridine synthase TruB bound to a T stem-loop RNA''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1K8W is a [ | + | 1K8W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K8W OCA]. |
==Reference== | ==Reference== | ||
| - | Cocrystal structure of a tRNA Psi55 pseudouridine synthase: nucleotide flipping by an RNA-modifying enzyme., Hoang C, Ferre-D'Amare AR, Cell. 2001 Dec 28;107(7):929-39. PMID:[http:// | + | Cocrystal structure of a tRNA Psi55 pseudouridine synthase: nucleotide flipping by an RNA-modifying enzyme., Hoang C, Ferre-D'Amare AR, Cell. 2001 Dec 28;107(7):929-39. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11779468 11779468] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Pseudouridylate synthase]] | [[Category: Pseudouridylate synthase]] | ||
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[[Category: trna]] | [[Category: trna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:14:04 2008'' |
Revision as of 10:14, 20 March 2008
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| , resolution 1.85Å | |||||||
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| Ligands: | |||||||
| Activity: | Pseudouridylate synthase, with EC number 4.2.1.70 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the E. coli pseudouridine synthase TruB bound to a T stem-loop RNA
Overview
Pseudouridine (Psi) synthases catalyze the isomerization of specific uridines in cellular RNAs to pseudouridines and may function as RNA chaperones. TruB is responsible for the Psi residue present in the T loops of virtually all tRNAs. The close homolog Cbf5/dyskerin is the catalytic subunit of box H/ACA snoRNPs. These carry out the pseudouridylation of eukaryotic rRNA and snRNAs. The 1.85 A resolution structure of TruB bound to RNA reveals that this enzyme recognizes the preformed three-dimensional structure of the T loop, primarily through shape complementarity. It accesses its substrate uridyl residue by flipping out the nucleotide and disrupts the tertiary structure of tRNA. Structural comparisons with TruB demonstrate that all Psi synthases are descended from a common molecular ancestor.
About this Structure
1K8W is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Cocrystal structure of a tRNA Psi55 pseudouridine synthase: nucleotide flipping by an RNA-modifying enzyme., Hoang C, Ferre-D'Amare AR, Cell. 2001 Dec 28;107(7):929-39. PMID:11779468
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