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4ap6

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[[Image:4ap6.png|left|200px]]
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==Crystal structure of human POFUT2 E54A mutant in complex with GDP- fucose==
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<StructureSection load='4ap6' size='340' side='right' caption='[[4ap6]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4ap6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AP6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AP6 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GFB:GUANOSINE-5-DIPHOSPHATE-BETA-L-FUCOPYRANOSE'>GFB</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ap5|4ap5]]</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptide-O-fucosyltransferase Peptide-O-fucosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.221 2.4.1.221] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ap6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ap6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ap6 RCSB], [http://www.ebi.ac.uk/pdbsum/4ap6 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Protein O-fucosylation is a post-translational modification found on serine/threonine residues of thrombospondin type 1 repeats (TSR). The fucose transfer is catalysed by the enzyme protein O-fucosyltransferase 2 (POFUT2) and &gt;40 human proteins contain the TSR consensus sequence for POFUT2-dependent fucosylation. To better understand O-fucosylation on TSR, we carried out a structural and functional analysis of human POFUT2 and its TSR substrate. Crystal structures of POFUT2 reveal a variation of the classical GT-B fold and identify sugar donor and TSR acceptor binding sites. Structural findings are correlated with steady-state kinetic measurements of wild-type and mutant POFUT2 and TSR and give insight into the catalytic mechanism and substrate specificity. By using an artificial mini-TSR substrate, we show that specificity is not primarily encoded in the TSR protein sequence but rather in the unusual 3D structure of a small part of the TSR. Our findings uncover that recognition of distinct conserved 3D fold motifs can be used as a mechanism to achieve substrate specificity by enzymes modifying completely folded proteins of very wide sequence diversity and biological function.
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{{STRUCTURE_4ap6| PDB=4ap6 | SCENE= }}
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Structure of human POFUT2: insights into thrombospondin type 1 repeat fold and O-fucosylation.,Chen CI, Keusch JJ, Klein D, Hess D, Hofsteenge J, Gut H EMBO J. 2012 May 15. doi: 10.1038/emboj.2012.143. PMID:22588082<ref>PMID:22588082</ref>
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===Crystal structure of human POFUT2 E54A mutant in complex with GDP- fucose===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_22588082}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[4ap6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AP6 OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:022588082</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Peptide-O-fucosyltransferase]]
[[Category: Peptide-O-fucosyltransferase]]
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[[Category: Chen, C.]]
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[[Category: Chen, C]]
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[[Category: Gut, H.]]
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[[Category: Gut, H]]
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[[Category: Hess, D.]]
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[[Category: Hess, D]]
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[[Category: Hofsteenge, J.]]
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[[Category: Hofsteenge, J]]
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[[Category: Keusch, J J.]]
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[[Category: Keusch, J J]]
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[[Category: Klein, D.]]
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[[Category: Klein, D]]
[[Category: Gt-b]]
[[Category: Gt-b]]
[[Category: Gt68]]
[[Category: Gt68]]
[[Category: Transferase]]
[[Category: Transferase]]

Revision as of 14:18, 4 January 2015

Crystal structure of human POFUT2 E54A mutant in complex with GDP- fucose

4ap6, resolution 3.40Å

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