1keq

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[[Image:1keq.jpg|left|200px]]<br /><applet load="1keq" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1keq.jpg|left|200px]]
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caption="1keq, resolution 1.88&Aring;" />
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'''Crystal Structure of F65A/Y131C Carbonic Anhydrase V, covalently modified with 4-chloromethylimidazole'''<br />
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{{Structure
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|PDB= 1keq |SIZE=350|CAPTION= <scene name='initialview01'>1keq</scene>, resolution 1.88&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=4MZ:4-METHYLIMIDAZOLE'>4MZ</scene> and <scene name='pdbligand=ACY:ACETIC ACID'>ACY</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1]
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|GENE= MCA5C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
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}}
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'''Crystal Structure of F65A/Y131C Carbonic Anhydrase V, covalently modified with 4-chloromethylimidazole'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1KEQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=K:'>K</scene>, <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=4MZ:'>4MZ</scene> and <scene name='pdbligand=ACY:'>ACY</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KEQ OCA].
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1KEQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KEQ OCA].
==Reference==
==Reference==
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Crystal structure of F65A/Y131C-methylimidazole carbonic anhydrase V reveals architectural features of an engineered proton shuttle., Jude KM, Wright SK, Tu C, Silverman DN, Viola RE, Christianson DW, Biochemistry. 2002 Feb 26;41(8):2485-91. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11851394 11851394]
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Crystal structure of F65A/Y131C-methylimidazole carbonic anhydrase V reveals architectural features of an engineered proton shuttle., Jude KM, Wright SK, Tu C, Silverman DN, Viola RE, Christianson DW, Biochemistry. 2002 Feb 26;41(8):2485-91. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11851394 11851394]
[[Category: Carbonate dehydratase]]
[[Category: Carbonate dehydratase]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: proton transfer]]
[[Category: proton transfer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:33:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:16:28 2008''

Revision as of 10:16, 20 March 2008

Image:1keq.jpg


PDB ID 1keq

Drag the structure with the mouse to rotate
, resolution 1.88Å
Ligands: , , and
Gene: MCA5C (Mus musculus)
Activity: Carbonate dehydratase, with EC number 4.2.1.1
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of F65A/Y131C Carbonic Anhydrase V, covalently modified with 4-chloromethylimidazole


Overview

The crystal structure of F65A/Y131C murine alpha-carbonic anhydrase V (CAV), covalently modified at cysteine residues with 4-chloromethylimidazole, is reported at 1.88 A resolution. This modification introduces a methylimidazole (MI) group at residue C131 in the active site with important consequences. F65A/Y131C-MI CAV exhibits an up to 3-fold enhancement of catalytic activity over that of wild-type CAV [Earnhardt, J. N., Wright, S. K., Qian, M., Tu, C., Laipis, P. J., Viola, R. E., and Silverman, D. N. (1999) Arch. Biochem. Biophys. 361, 264-270]. In this modified CAV variant, C131-MI acts as a proton shuttle, facilitating the deprotonation of a zinc-bound water molecule to regenerate the nucleophilic zinc-bound hydroxide ion. A network of three hydrogen-bonded water molecules, across which proton transfer likely proceeds, bridges the zinc-bound water molecule and the C131-MI imidazole group. The structure of F65A/Y131C-MI CAV is compared to structures of Y64H/F65A murine CAV, wild-type human alpha-carbonic anhydrase II, and the gamma-carbonic anhydrase from Methanosarcina thermophilain an effort to outline common features of catalytic proton shuttles.

About this Structure

1KEQ is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Crystal structure of F65A/Y131C-methylimidazole carbonic anhydrase V reveals architectural features of an engineered proton shuttle., Jude KM, Wright SK, Tu C, Silverman DN, Viola RE, Christianson DW, Biochemistry. 2002 Feb 26;41(8):2485-91. PMID:11851394

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