1kev

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1kev.gif|left|200px]]<br /><applet load="1kev" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1kev.gif|left|200px]]
-
caption="1kev, resolution 2.05&Aring;" />
+
 
-
'''STRUCTURE OF NADP-DEPENDENT ALCOHOL DEHYDROGENASE'''<br />
+
{{Structure
 +
|PDB= 1kev |SIZE=350|CAPTION= <scene name='initialview01'>1kev</scene>, resolution 2.05&Aring;
 +
|SITE= <scene name='pdbsite=S1:Catalytic+Site'>S1</scene>, <scene name='pdbsite=S2:Catalytic+Site'>S2</scene>, <scene name='pdbsite=S3:Catalytic+Site'>S3</scene> and <scene name='pdbsite=S4:Catalytic+Site'>S4</scene>
 +
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=NDP:NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE'>NDP</scene>
 +
|ACTIVITY= [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase_(NADP(+)) Alcohol dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.2 1.1.1.2]
 +
|GENE=
 +
}}
 +
 
 +
'''STRUCTURE OF NADP-DEPENDENT ALCOHOL DEHYDROGENASE'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1KEV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_beijerinckii Clostridium beijerinckii] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=NDP:'>NDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase_(NADP(+)) Alcohol dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.2 1.1.1.2] Known structural/functional Sites: <scene name='pdbsite=S1:Catalytic+Site'>S1</scene>, <scene name='pdbsite=S2:Catalytic+Site'>S2</scene>, <scene name='pdbsite=S3:Catalytic+Site'>S3</scene> and <scene name='pdbsite=S4:Catalytic+Site'>S4</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KEV OCA].
+
1KEV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_beijerinckii Clostridium beijerinckii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KEV OCA].
==Reference==
==Reference==
-
Crystalline alcohol dehydrogenases from the mesophilic bacterium Clostridium beijerinckii and the thermophilic bacterium Thermoanaerobium brockii: preparation, characterization and molecular symmetry., Korkhin Y, Frolow F, Bogin O, Peretz M, Kalb AJ, Burstein Y, Acta Crystallogr D Biol Crystallogr. 1996 Jul 1;52(Pt 4):882-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299659 15299659]
+
Crystalline alcohol dehydrogenases from the mesophilic bacterium Clostridium beijerinckii and the thermophilic bacterium Thermoanaerobium brockii: preparation, characterization and molecular symmetry., Korkhin Y, Frolow F, Bogin O, Peretz M, Kalb AJ, Burstein Y, Acta Crystallogr D Biol Crystallogr. 1996 Jul 1;52(Pt 4):882-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15299659 15299659]
[[Category: Alcohol dehydrogenase (NADP(+))]]
[[Category: Alcohol dehydrogenase (NADP(+))]]
[[Category: Clostridium beijerinckii]]
[[Category: Clostridium beijerinckii]]
Line 22: Line 31:
[[Category: zinc]]
[[Category: zinc]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:33:23 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:16:30 2008''

Revision as of 10:16, 20 March 2008

Image:1kev.gif


PDB ID 1kev

Drag the structure with the mouse to rotate
, resolution 2.05Å
Sites: , , and
Ligands: and
Activity: Alcohol dehydrogenase (NADP(+)), with EC number 1.1.1.2
Coordinates: save as pdb, mmCIF, xml



STRUCTURE OF NADP-DEPENDENT ALCOHOL DEHYDROGENASE


Overview

Two tetrameric NADP(+)-dependent bacterial secondary alcohol dehydrogenases have been crystallized in the apo- and the holo-enzyme forms. Crystals of the holo-enzyme from the mesophilic Clostridium beijerinckii (NCBAD) belong to space group P2(1)2(1)2(1) with unit-cell dimensions a = 90.5, b = 127.9, c = 151.4 A. Crystals of the apo-enzyme (CBAD) belong to the same space group with unit-cell dimensions a = 80.4, b = 102.3, c = 193.5 A. Crystals of the holo-enzyme from the thermophilic Thermoanaerobium brockii (NTBAD) belong to space group P6(1(5)) (a = b = 80.6, c = 400.7 A). Crystals of the apo-form of TBAD (point mutant GI98D) belong to space group P2(1) with cell dimensions a = 123.0, b = 84.8, c = 160.4 A beta = 99.5 degrees. Crystals of CBAD, NCBAD and NTBAD contain one tetramer per asymmetric unit. They diffract to 2.0 A resolution at liquid nitrogen temperature. Crystals of TBAD(GI98D) have two tetramers per asymmetric unit and diffract to 2.7 A at 276 K. Self-rotation analysis shows that both enzymes are tetramers of 222 symmetry.

About this Structure

1KEV is a Single protein structure of sequence from Clostridium beijerinckii. Full crystallographic information is available from OCA.

Reference

Crystalline alcohol dehydrogenases from the mesophilic bacterium Clostridium beijerinckii and the thermophilic bacterium Thermoanaerobium brockii: preparation, characterization and molecular symmetry., Korkhin Y, Frolow F, Bogin O, Peretz M, Kalb AJ, Burstein Y, Acta Crystallogr D Biol Crystallogr. 1996 Jul 1;52(Pt 4):882-6. PMID:15299659

Page seeded by OCA on Thu Mar 20 12:16:30 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1kev"
Personal tools