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1kf5
From Proteopedia
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| - | [[Image:1kf5.jpg|left|200px]] | + | [[Image:1kf5.jpg|left|200px]] |
| - | + | ||
| - | '''Atomic Resolution Structure of RNase A at pH 7.1''' | + | {{Structure |
| + | |PDB= 1kf5 |SIZE=350|CAPTION= <scene name='initialview01'>1kf5</scene>, resolution 1.15Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Atomic Resolution Structure of RNase A at pH 7.1''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1KF5 is a [ | + | 1KF5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KF5 OCA]. |
==Reference== | ==Reference== | ||
| - | Atomic resolution structures of ribonuclease A at six pH values., Berisio R, Sica F, Lamzin VS, Wilson KS, Zagari A, Mazzarella L, Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):441-50. Epub 2002, Feb 21. PMID:[http:// | + | Atomic resolution structures of ribonuclease A at six pH values., Berisio R, Sica F, Lamzin VS, Wilson KS, Zagari A, Mazzarella L, Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):441-50. Epub 2002, Feb 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11856829 11856829] |
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Pancreatic ribonuclease]] | [[Category: Pancreatic ribonuclease]] | ||
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[[Category: crystal]] | [[Category: crystal]] | ||
[[Category: ph]] | [[Category: ph]] | ||
| - | [[Category: rnase | + | [[Category: rnase some]] |
[[Category: soaking]] | [[Category: soaking]] | ||
[[Category: titration]] | [[Category: titration]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:16:36 2008'' |
Revision as of 10:16, 20 March 2008
| |||||||
| , resolution 1.15Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | Pancreatic ribonuclease, with EC number 3.1.27.5 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Atomic Resolution Structure of RNase A at pH 7.1
Overview
The diffraction pattern of protein crystals extending to atomic resolution guarantees a very accurate picture of the molecular structure and enables the study of subtle phenomena related to protein functionality. Six structures of bovine pancreatic ribonuclease at the pH* values 5.2, 5.9, 6.3, 7.1, 8.0 and 8.8 and at resolution limits in the range 1.05-1.15A have been refined. An overall description of the six structures and several aspects, mainly regarding pH-triggered conformational changes, are described here. Since subtle variations were expected, a thorough validation assessment of the six refined models was first carried out. Some stereochemical parameters, such as the N[bond]C(alpha)[bond]C angle and the pyramidalization at the carbonyl C atoms, indicate that the standard target values and their weights typically used in refinement may need revision. A detailed comparison of the six structures has provided experimental evidence on the role of Lys41 in catalysis. Furthermore, insights are given into the structural effects related to the pH-dependent binding of a sulfate anion, which mimics the phosphate group of RNA, in the active site. Finally, the results support a number of thermodynamic and kinetic experimental data concerning the role of the disulfide bridge between Cys65 and Cys72 in the folding of RNase A.
About this Structure
1KF5 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Atomic resolution structures of ribonuclease A at six pH values., Berisio R, Sica F, Lamzin VS, Wilson KS, Zagari A, Mazzarella L, Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):441-50. Epub 2002, Feb 21. PMID:11856829
Page seeded by OCA on Thu Mar 20 12:16:36 2008
Categories: Bos taurus | Pancreatic ribonuclease | Single protein | Berisio, R. | Lamzin, V S. | Mazzarella, L. | Sica, F. | Wilson, K S. | Zagari, A. | Crystal | Ph | Rnase some | Soaking | Titration
