1khp
From Proteopedia
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| - | [[Image:1khp.jpg|left|200px]] | + | [[Image:1khp.jpg|left|200px]] |
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| - | '''Monoclinic form of papain/ZLFG-DAM covalent complex''' | + | {{Structure |
| + | |PDB= 1khp |SIZE=350|CAPTION= <scene name='initialview01'>1khp</scene>, resolution 2.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Papain Papain], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.2 3.4.22.2] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Monoclinic form of papain/ZLFG-DAM covalent complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1KHP is a [ | + | 1KHP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Carica_papaya Carica papaya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHP OCA]. |
==Reference== | ==Reference== | ||
| - | Two polymorphs of a covalent complex between papain and a diazomethylketone inhibitor., Janowski R, Kozak M, Jankowska E, Grzonka Z, Jaskolski M, J Pept Res. 2004 Oct;64(4):141-50. PMID:[http:// | + | Two polymorphs of a covalent complex between papain and a diazomethylketone inhibitor., Janowski R, Kozak M, Jankowska E, Grzonka Z, Jaskolski M, J Pept Res. 2004 Oct;64(4):141-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15357669 15357669] |
[[Category: Carica papaya]] | [[Category: Carica papaya]] | ||
[[Category: Papain]] | [[Category: Papain]] | ||
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[[Category: protease inhibitor]] | [[Category: protease inhibitor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:17:37 2008'' |
Revision as of 10:17, 20 March 2008
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| , resolution 2.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | Papain, with EC number 3.4.22.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Monoclinic form of papain/ZLFG-DAM covalent complex
Overview
The three-dimensional structure of two polymorphs of a ZLFG-CH2-papain covalent complex has been determined by X-ray crystallography. The structures indicate that: (i) the methylene carbon atom of the inhibitor is covalently bound to the Sgamma atom of Cys25 of papain; (ii) the hydrophobic S2 pocket formed by Pro68, Val133, Val157, and Asp158 is occupied by the inhibitor's phenylalanyl P2 side chain; (iii) extensive hydrogen bonding and hydrophobic interactions are responsible for the interaction of the inhibitor with the enzyme. Comparison with similar structures suggests that in covalent complexes preservation of main chain-main chain interactions between the enzyme and the inhibitor may have higher priority than the P-S interactions.
About this Structure
1KHP is a Single protein structure of sequence from Carica papaya. Full crystallographic information is available from OCA.
Reference
Two polymorphs of a covalent complex between papain and a diazomethylketone inhibitor., Janowski R, Kozak M, Jankowska E, Grzonka Z, Jaskolski M, J Pept Res. 2004 Oct;64(4):141-50. PMID:15357669
Page seeded by OCA on Thu Mar 20 12:17:37 2008
