3o5w
From Proteopedia
(Difference between revisions)
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| - | [[ | + | ==Binding of kinetin in the active site of mistletoe lectin I== |
| + | <StructureSection load='3o5w' size='340' side='right' caption='[[3o5w]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3o5w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Viscum_album Viscum album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O5W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O5W FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=H35:N-(FURAN-2-YLMETHYL)-7H-PURIN-6-AMINE'>H35</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1m2t|1m2t]], [[2r9k|2r9k]], [[3d7w|3d7w]]</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o5w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o5w RCSB], [http://www.ebi.ac.uk/pdbsum/3o5w PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The crystal structure of the ribosome inhibiting protein Mistletoe Lectin I (ML-I) derived from the European mistletoe, Viscum album, in complex with kinetin has been refined at 2.7A resolution. Suitably large crystals of ML-I were obtained applying the counter diffusion method using the Gel Tube R Crystallization Kit (GT-R) on board the Russian Service Module on the international space station ISS within the GCF mission No. 6, arranged by the Japanese aerospace exploration agency (JAXA). Hexagonal bi-pyramidal crystals were grown during three months under microgravity. Before data collection the crystals were soaked in a saturated solution of kinetin and diffraction data to 2.7A were collected using synchrotron radiation and cryogenic techniques. The atomic model was refined and revealed a single kinetin molecule in the ribosome inactivation site of ML-I. The complex demonstrates the feasibility of mistletoe to bind plant hormones out of the host regulation system as part of a self protection mechanism. | ||
| - | + | Binding of the plant hormone kinetin in the active site of Mistletoe Lectin I from Viscum album.,Malecki PH, Rypniewski W, Szymanski M, Barciszewski J, Meyer A Biochim Biophys Acta. 2012 Feb;1824(2):334-8. doi: 10.1016/j.bbapap.2011.10.013. , Epub 2011 Oct 28. PMID:22064121<ref>PMID:22064121</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | + | </StructureSection> | |
[[Category: Viscum album]] | [[Category: Viscum album]] | ||
[[Category: RRNA N-glycosylase]] | [[Category: RRNA N-glycosylase]] | ||
| - | [[Category: Barciszewski, J | + | [[Category: Barciszewski, J]] |
| - | [[Category: Betzel, C | + | [[Category: Betzel, C]] |
| - | [[Category: Malecki, P H | + | [[Category: Malecki, P H]] |
| - | [[Category: Meyer, A | + | [[Category: Meyer, A]] |
| - | [[Category: Rypniewski, W | + | [[Category: Rypniewski, W]] |
| - | [[Category: Szymanski, M | + | [[Category: Szymanski, M]] |
[[Category: Active site]] | [[Category: Active site]] | ||
[[Category: Cytokinin]] | [[Category: Cytokinin]] | ||
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[[Category: Microgravity]] | [[Category: Microgravity]] | ||
[[Category: Ribosome inactivating protein]] | [[Category: Ribosome inactivating protein]] | ||
| - | [[Category: Viscum album]] | ||
Revision as of 10:50, 9 December 2014
Binding of kinetin in the active site of mistletoe lectin I
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