1ko7
From Proteopedia
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| - | [[Image:1ko7.gif|left|200px]] | + | [[Image:1ko7.gif|left|200px]] |
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| - | '''X-ray structure of the HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution''' | + | {{Structure |
| + | |PDB= 1ko7 |SIZE=350|CAPTION= <scene name='initialview01'>1ko7</scene>, resolution 1.95Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= HPRK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1288 Staphylococcus xylosus]) | ||
| + | }} | ||
| + | |||
| + | '''X-ray structure of the HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1KO7 is a [ | + | 1KO7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_xylosus Staphylococcus xylosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KO7 OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the full-length HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution: Mimicking the product/substrate of the phospho transfer reactions., Marquez JA, Hasenbein S, Koch B, Fieulaine S, Nessler S, Russell RB, Hengstenberg W, Scheffzek K, Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3458-63. PMID:[http:// | + | Structure of the full-length HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution: Mimicking the product/substrate of the phospho transfer reactions., Marquez JA, Hasenbein S, Koch B, Fieulaine S, Nessler S, Russell RB, Hengstenberg W, Scheffzek K, Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3458-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11904409 11904409] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Staphylococcus xylosus]] | [[Category: Staphylococcus xylosus]] | ||
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[[Category: substrate]] | [[Category: substrate]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:19:58 2008'' |
Revision as of 10:20, 20 March 2008
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| , resolution 1.95Å | |||||||
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| Ligands: | |||||||
| Gene: | HPRK (Staphylococcus xylosus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
X-ray structure of the HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution
Overview
The histidine containing phospho carrier protein (HPr) kinase/phosphatase is involved in carbon catabolite repression, mainly in Gram-positive bacteria. It is a bifunctional enzyme that phosphorylates Ser-46-HPr in an ATP-dependent reaction and dephosphorylates P-Ser-46-HPr. X-ray analysis of the full-length crystalline enzyme from Staphylococcus xylosus at a resolution of 1.95 A shows the enzyme to consist of two clearly separated domains that are assembled in a hexameric structure resembling a three-bladed propeller. The N-terminal domain has a betaalphabeta fold similar to a segment from enzyme I of the sugar phosphotransferase system and to the uridyl-binding portion of MurF; it is structurally organized in three dimeric modules exposed to form the propeller blades. Two unexpected phosphate ions associated with highly conserved residues were found in the N-terminal dimeric interface. The C-terminal kinase domain is similar to that of the Lactobacillus casei enzyme and is assembled in six copies to form the compact central hub of the propeller. Beyond previously reported similarity with adenylate kinase, we suggest evolutionary relationship with phosphoenolpyruvate carboxykinase. In addition to a phosphate ion in the phosphate-binding loop of the kinase domain, we have identified a second phosphate-binding site that, by comparison with adenylate kinases, we believe accommodates a product/substrate phosphate, normally covalently linked to Ser-46 of HPr. Thus, we propose that our structure represents a product/substrate mimic of the kinase/phosphatase reaction.
About this Structure
1KO7 is a Single protein structure of sequence from Staphylococcus xylosus. Full crystallographic information is available from OCA.
Reference
Structure of the full-length HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution: Mimicking the product/substrate of the phospho transfer reactions., Marquez JA, Hasenbein S, Koch B, Fieulaine S, Nessler S, Russell RB, Hengstenberg W, Scheffzek K, Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3458-63. PMID:11904409
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