1kuj
From Proteopedia
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| - | [[Image:1kuj.gif|left|200px]] | + | [[Image:1kuj.gif|left|200px]] |
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| - | '''Crystal structure of Jacalin complexed with 1-O-methyl-alpha-D-mannose''' | + | {{Structure |
| + | |PDB= 1kuj |SIZE=350|CAPTION= <scene name='initialview01'>1kuj</scene>, resolution 2.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of Jacalin complexed with 1-O-methyl-alpha-D-mannose''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1KUJ is a [ | + | 1KUJ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Artocarpus_integer Artocarpus integer]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KUJ OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for the unusual carbohydrate-binding specificity of jacalin towards galactose and mannose., Bourne Y, Astoul CH, Zamboni V, Peumans WJ, Menu-Bouaouiche L, Van Damme EJ, Barre A, Rouge P, Biochem J. 2002 May 15;364(Pt 1):173-80. PMID:[http:// | + | Structural basis for the unusual carbohydrate-binding specificity of jacalin towards galactose and mannose., Bourne Y, Astoul CH, Zamboni V, Peumans WJ, Menu-Bouaouiche L, Van Damme EJ, Barre A, Rouge P, Biochem J. 2002 May 15;364(Pt 1):173-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11988090 11988090] |
[[Category: Artocarpus integer]] | [[Category: Artocarpus integer]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: lectin]] | [[Category: lectin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:22:16 2008'' |
Revision as of 10:22, 20 March 2008
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| , resolution 2.00Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Jacalin complexed with 1-O-methyl-alpha-D-mannose
Overview
Evidence is presented that the specificity of jacalin, the seed lectin from jack fruit (Artocarpus integrifolia), is not directed exclusively against the T-antigen disaccharide Galbeta1,3GalNAc, lactose and galactose, but also against mannose and oligomannosides. Biochemical analyses based on surface-plasmon-resonance measurements, combined with the X-ray-crystallographic determination of the structure of a jacalin-alpha-methyl-mannose complex at 2 A resolution, demonstrated clearly that jacalin is fully capable of binding mannose. Besides mannose, jacalin also interacts readily with glucose, N-acetylneuraminic acid and N-acetylmuramic acid. Structural analyses demonstrated that the relatively large size of the carbohydrate-binding site enables jacalin to accommodate monosaccharides with different hydroxyl conformations and provided unambiguous evidence that the beta-prism structure of jacalin is a sufficiently flexible structural scaffold to confer different carbohydrate-binding specificities to a single lectin.
About this Structure
1KUJ is a Protein complex structure of sequences from Artocarpus integer. Full crystallographic information is available from OCA.
Reference
Structural basis for the unusual carbohydrate-binding specificity of jacalin towards galactose and mannose., Bourne Y, Astoul CH, Zamboni V, Peumans WJ, Menu-Bouaouiche L, Van Damme EJ, Barre A, Rouge P, Biochem J. 2002 May 15;364(Pt 1):173-80. PMID:11988090
Page seeded by OCA on Thu Mar 20 12:22:16 2008
