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1l2f

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[[Image:1l2f.gif|left|200px]]<br /><applet load="1l2f" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1l2f.gif|left|200px]]
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caption="1l2f, resolution 2.50&Aring;" />
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'''Crystal structure of NusA from Thermotoga maritima: a structure-based role of the N-terminal domain'''<br />
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{{Structure
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|PDB= 1l2f |SIZE=350|CAPTION= <scene name='initialview01'>1l2f</scene>, resolution 2.50&Aring;
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|SITE=
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'''Crystal structure of NusA from Thermotoga maritima: a structure-based role of the N-terminal domain'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1L2F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2F OCA].
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1L2F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2F OCA].
==Reference==
==Reference==
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Crystal structure of NusA from Thermotoga maritima and functional implication of the N-terminal domain., Shin DH, Nguyen HH, Jancarik J, Yokota H, Kim R, Kim SH, Biochemistry. 2003 Nov 25;42(46):13429-37. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14621988 14621988]
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Crystal structure of NusA from Thermotoga maritima and functional implication of the N-terminal domain., Shin DH, Nguyen HH, Jancarik J, Yokota H, Kim R, Kim SH, Biochemistry. 2003 Nov 25;42(46):13429-37. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14621988 14621988]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
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[[Category: psi]]
[[Category: psi]]
[[Category: rna polymerase]]
[[Category: rna polymerase]]
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[[Category: structural genomics]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:40:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:25:24 2008''

Revision as of 10:25, 20 March 2008

Image:1l2f.gif


PDB ID 1l2f

Drag the structure with the mouse to rotate
, resolution 2.50Å
Coordinates: save as pdb, mmCIF, xml



Crystal structure of NusA from Thermotoga maritima: a structure-based role of the N-terminal domain


Overview

We report the crystal structure of N-utilizing substance A protein (NusA) from Thermotoga maritima (TmNusA), a protein involved in transcriptional pausing, termination, and antitermination. TmNusA has an elongated rod-shaped structure consisting of an N-terminal domain (NTD, residues 1-132) and three RNA binding domains (RBD). The NTD consists of two subdomains, the globular head and the helical body domains, that comprise a unique three-dimensional structure that may be important for interacting with RNA polymerase. The globular head domain possesses a high content of negatively charged residues that may interact with the positively charged flaplike domain of RNA polymerase. The helical body domain is composed of a three-helix bundle that forms a hydrophobic core with the aid of two neighboring beta-strands. This domain shows structural similarity with one of the helical domains of sigma(70) factor from Escherichia coli. One side of the molecular surface shows positive electrostatic potential suitable for nonspecific RNA interaction. The RBD is composed of one S1 domain and two K-homology (KH) domains forming an elongated RNA binding surface. Structural comparison between TmNusA and Mycobacterium tuberculosis NusA reveals a possible hinge motion between NTD and RBD. In addition, a functional implication of the NTD in its interaction with RNA polymerase is discussed.

About this Structure

1L2F is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.

Reference

Crystal structure of NusA from Thermotoga maritima and functional implication of the N-terminal domain., Shin DH, Nguyen HH, Jancarik J, Yokota H, Kim R, Kim SH, Biochemistry. 2003 Nov 25;42(46):13429-37. PMID:14621988

Page seeded by OCA on Thu Mar 20 12:25:24 2008

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