3f5t
From Proteopedia
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| - | [[ | + | ==X-ray Structure of H5N1 NS1== |
| + | <StructureSection load='3f5t' size='340' side='right' caption='[[3f5t]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3f5t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Unidentified_influenza_virus Unidentified influenza virus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3eu6 3eu6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F5T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3F5T FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2gx9|2gx9]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3f5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f5t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3f5t RCSB], [http://www.ebi.ac.uk/pdbsum/3f5t PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The recent emergence of highly pathogenic avian (H5N1) influenza viruses, their epizootic and panzootic nature, and their association with lethal human infections have raised significant global health concerns. Several studies have underlined the importance of non-structural protein NS1 in the increased pathogenicity and virulence of these strains. NS1, which consists of two domains-a double-stranded RNA (dsRNA) binding domain and the effector domain, separated through a linker-is an antagonist of antiviral type-I interferon response in the host. Here we report the X-ray structure of the full-length NS1 from an H5N1 strain (A/Vietnam/1203/2004) that was associated with 60% of human deaths in an outbreak in Vietnam. Compared to the individually determined structures of the RNA binding domain and the effector domain from non-H5N1 strains, the RNA binding domain within H5N1 NS1 exhibits modest structural changes, while the H5N1 effector domain shows significant alteration, particularly in the dimeric interface. Although both domains in the full-length NS1 individually participate in dimeric interactions, an unexpected finding is that these interactions result in the formation of a chain of NS1 molecules instead of distinct dimeric units. Three such chains in the crystal interact with one another extensively to form a tubular organization of similar dimensions to that observed in the cryo-electron microscopy images of NS1 in the presence of dsRNA. The tubular oligomeric organization of NS1, in which residues implicated in dsRNA binding face a 20-A-wide central tunnel, provides a plausible mechanism for how NS1 sequesters varying lengths of dsRNA, to counter cellular antiviral dsRNA response pathways, while simultaneously interacting with other cellular ligands during an infection. | ||
| - | + | X-ray structure of NS1 from a highly pathogenic H5N1 influenza virus.,Bornholdt ZA, Prasad BV Nature. 2008 Nov 5. PMID:18987632<ref>PMID:18987632</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
*[[Nonstructural protein|Nonstructural protein]] | *[[Nonstructural protein|Nonstructural protein]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Unidentified influenza virus]] | [[Category: Unidentified influenza virus]] | ||
| - | [[Category: Bornholdt, Z A | + | [[Category: Bornholdt, Z A]] |
| - | [[Category: Prasad, B V.V | + | [[Category: Prasad, B V.V]] |
[[Category: H5n1]] | [[Category: H5n1]] | ||
[[Category: Host-virus interaction]] | [[Category: Host-virus interaction]] | ||
Revision as of 08:46, 26 November 2014
X-ray Structure of H5N1 NS1
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