1l9v
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1l9v.jpg|left|200px]] | + | [[Image:1l9v.jpg|left|200px]] |
| - | + | ||
| - | '''Non Structural protein encoded by gene segment 8 of rotavirus (NSP2), an NTPase, ssRNA binding and nucleic acid helix-destabilizing protein''' | + | {{Structure |
| + | |PDB= 1l9v |SIZE=350|CAPTION= <scene name='initialview01'>1l9v</scene>, resolution 2.60Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= gene segment 8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Simian 11 rotavirus A]) | ||
| + | }} | ||
| + | |||
| + | '''Non Structural protein encoded by gene segment 8 of rotavirus (NSP2), an NTPase, ssRNA binding and nucleic acid helix-destabilizing protein''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1L9V is a [ | + | 1L9V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Simian_11_rotavirus_a Simian 11 rotavirus a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L9V OCA]. |
==Reference== | ==Reference== | ||
| - | Rotavirus protein involved in genome replication and packaging exhibits a HIT-like fold., Jayaram H, Taraporewala Z, Patton JT, Prasad BV, Nature. 2002 May 16;417(6886):311-5. PMID:[http:// | + | Rotavirus protein involved in genome replication and packaging exhibits a HIT-like fold., Jayaram H, Taraporewala Z, Patton JT, Prasad BV, Nature. 2002 May 16;417(6886):311-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12015608 12015608] |
[[Category: Simian 11 rotavirus a]] | [[Category: Simian 11 rotavirus a]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 22: | Line 31: | ||
[[Category: two domain protein]] | [[Category: two domain protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:28:19 2008'' |
Revision as of 10:28, 20 March 2008
| |||||||
| , resolution 2.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | gene segment 8 (Simian 11 rotavirus A) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Non Structural protein encoded by gene segment 8 of rotavirus (NSP2), an NTPase, ssRNA binding and nucleic acid helix-destabilizing protein
Overview
Rotavirus, the major cause of life-threatening infantile gastroenteritis, is a member of the Reoviridae. Although the structures of rotavirus and other members of the Reoviridae have been extensively studied, little is known about the structures of virus-encoded non-structural proteins that are essential for genome replication and packaging. The non-structural protein NSP2 of rotavirus, which exhibits nucleoside triphosphatase, single-stranded RNA binding, and nucleic-acid helix-destabilizing activities, is a major component of viral replicase complexes. We present here the X-ray structure of the functional octamer of NSP2 determined to a resolution of 2.6 A. The NSP2 monomer has two distinct domains. The amino-terminal domain has a new fold. The carboxy-terminal domain resembles the ubiquitous cellular histidine triad (HIT) group of nucleotidyl hydrolases. This structural similarity suggests that the nucleotide-binding site is located inside the cleft between the two domains. Prominent grooves that run diagonally across the doughnut-shaped octamer are probable locations for RNA binding. Several RNA binding sites, resulting from the quaternary organization of NSP2 monomers, may be required for the helix destabilizing activity of NSP2 and its function during genome replication and packaging.
About this Structure
1L9V is a Single protein structure of sequence from Simian 11 rotavirus a. Full crystallographic information is available from OCA.
Reference
Rotavirus protein involved in genome replication and packaging exhibits a HIT-like fold., Jayaram H, Taraporewala Z, Patton JT, Prasad BV, Nature. 2002 May 16;417(6886):311-5. PMID:12015608
Page seeded by OCA on Thu Mar 20 12:28:19 2008
