3zv2

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[[Image:3zv2.png|left|200px]]
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==HUMAN PROTEIN-TYROSINE PHOSPHATASE 1B C215A, S216A MUTANT==
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<StructureSection load='3zv2' size='340' side='right' caption='[[3zv2]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3zv2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZV2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZV2 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2f6t|2f6t]], [[2f6f|2f6f]], [[2cnf|2cnf]], [[2cm3|2cm3]], [[1g1f|1g1f]], [[2cm2|2cm2]], [[1q6n|1q6n]], [[1ptu|1ptu]], [[1c84|1c84]], [[2veu|2veu]], [[1nz7|1nz7]], [[1q1m|1q1m]], [[2azr|2azr]], [[1c88|1c88]], [[1oet|1oet]], [[1c83|1c83]], [[1sug|1sug]], [[1onz|1onz]], [[1g1g|1g1g]], [[1xbo|1xbo]], [[1ptv|1ptv]], [[2cnh|2cnh]], [[2hnp|2hnp]], [[1nwl|1nwl]], [[1t49|1t49]], [[2f6y|2f6y]], [[2cne|2cne]], [[1nl9|1nl9]], [[1c86|1c86]], [[1kav|1kav]], [[2hnq|2hnq]], [[1jf7|1jf7]], [[1pxh|1pxh]], [[1q6s|1q6s]], [[1l8g|1l8g]], [[2cma|2cma]], [[1q6m|1q6m]], [[1bzj|1bzj]], [[1i57|1i57]], [[1wax|1wax]], [[1ony|1ony]], [[1g1h|1g1h]], [[1kak|1kak]], [[2cng|2cng]], [[1t4j|1t4j]], [[2cni|2cni]], [[2b07|2b07]], [[2cm8|2cm8]], [[1eeo|1eeo]], [[1g7g|1g7g]], [[1ptt|1ptt]], [[1qxk|1qxk]], [[1ecv|1ecv]], [[1oem|1oem]], [[1nwe|1nwe]], [[2cmb|2cmb]], [[2fjn|2fjn]], [[1aax|1aax]], [[2vev|2vev]], [[2vey|2vey]], [[2b4s|2b4s]], [[2cm7|2cm7]], [[2bgd|2bgd]], [[1a5y|1a5y]], [[2vex|2vex]], [[1c85|1c85]], [[2cmc|2cmc]], [[2vew|2vew]], [[1lqf|1lqf]], [[2f6w|2f6w]], [[1c87|1c87]], [[1no6|1no6]], [[1pyn|1pyn]], [[2bge|2bge]], [[1oes|1oes]], [[1bzc|1bzc]], [[2f6z|2f6z]], [[2f71|2f71]], [[1oeu|1oeu]], [[1q6t|1q6t]], [[1g7f|1g7f]], [[1nny|1nny]], [[1q6j|1q6j]], [[2f70|2f70]], [[1q6p|1q6p]], [[2f6v|2f6v]], [[1t48|1t48]], [[1oeo|1oeo]], [[2fjm|2fjm]], [[1pa1|1pa1]], [[1ph0|1ph0]], [[1bzh|1bzh]], [[1gfy|1gfy]], [[1een|1een]], [[1pty|1pty]], [[1oev|1oev]]</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zv2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zv2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zv2 RCSB], [http://www.ebi.ac.uk/pdbsum/3zv2 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Protein tyrosine phosphatase 1B (PTP1B) plays important roles in downregulation of insulin and leptin signaling and is an established therapeutic target for diabetes and obesity. PTP1B is regulated by reactive oxygen species (ROS) produced in response to various stimuli, including insulin. The reversibly oxidized form of the enzyme (PTP1B-OX) is inactive and undergoes profound conformational changes at the active site. We generated conformation-sensor antibodies, in the form of single-chain variable fragments (scFvs), that stabilize PTP1B-OX and thereby inhibit its phosphatase function. Expression of conformation-sensor scFvs as intracellular antibodies (intrabodies) enhanced insulin-induced tyrosyl phosphorylation of the beta subunit of the insulin receptor and its substrate IRS-1 and increased insulin-induced phosphorylation of PKB/AKT. Our data suggest that stabilization of the oxidized, inactive form of PTP1B with appropriate therapeutic molecules may offer a paradigm for phosphatase drug development.
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{{STRUCTURE_3zv2| PDB=3zv2 | SCENE= }}
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Conformation-Sensing Antibodies Stabilize the Oxidized Form of PTP1B and Inhibit Its Phosphatase Activity.,Haque A, Andersen JN, Salmeen A, Barford D, Tonks NK Cell. 2011 Sep 30;147(1):185-98. PMID:21962515<ref>PMID:21962515</ref>
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===HUMAN PROTEIN-TYROSINE PHOSPHATASE 1B C215A, S216A MUTANT===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_21962515}}
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==About this Structure==
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[[3zv2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZV2 OCA].
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==See Also==
==See Also==
*[[Tyrosine phosphatase|Tyrosine phosphatase]]
*[[Tyrosine phosphatase|Tyrosine phosphatase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:021962515</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein-tyrosine-phosphatase]]
[[Category: Protein-tyrosine-phosphatase]]
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[[Category: Barford, D.]]
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[[Category: Barford, D]]
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[[Category: Salmeen, A.]]
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[[Category: Salmeen, A]]
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[[Category: Tonks, N K.]]
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[[Category: Tonks, N K]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Phosphorylation]]
[[Category: Phosphorylation]]

Revision as of 17:39, 9 December 2014

HUMAN PROTEIN-TYROSINE PHOSPHATASE 1B C215A, S216A MUTANT

3zv2, resolution 2.80Å

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