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2k48

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[[Image:2k48.png|left|200px]]
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==NMR Structure of the N-terminal Coiled Coil Domain of the Andes Hantavirus Nucleocapsid Protein==
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<StructureSection load='2k48' size='340' side='right' caption='[[2k48]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2k48]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Andes_virus Andes virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K48 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K48 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k48 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2k48 RCSB], [http://www.ebi.ac.uk/pdbsum/2k48 PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k4/2k48_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The hantaviruses are emerging infectious viruses that in humans can cause a cardiopulmonary syndrome or a hemorrhagic fever with renal syndrome. The nucleocapsid (N) is the most abundant viral protein, and during viral assembly, the N protein forms trimers and packages the viral RNA genome. Here, we report the NMR structure of the N-terminal domain (residues 1-74, called N1-74) of the Andes hantavirus N protein. N1-74 forms two long helices (alpha1 and alpha2) that intertwine into a coiled coil domain. The conserved hydrophobic residues at the helix alpha1-alpha2 interface stabilize the coiled coil; however, there are many conserved surface residues whose function is not known. Site-directed mutagenesis, CD spectroscopy, and immunocytochemistry reveal that a point mutation in the conserved basic surface formed by Arg22 or Lys26 lead to antibody recognition based on the subcellular localization of the N protein. Thus, Arg22 and Lys26 are likely involved in a conformational change or molecular recognition when the N protein is trafficked from the cytoplasm to the Golgi, the site of viral assembly and maturation.
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{{STRUCTURE_2k48| PDB=2k48 | SCENE= }}
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NMR structure of the N-terminal coiled coil domain of the Andes hantavirus nucleocapsid protein.,Wang Y, Boudreaux DM, Estrada DF, Egan CW, St Jeor SC, De Guzman RN J Biol Chem. 2008 Oct 17;283(42):28297-304. Epub 2008 Aug 7. PMID:18687679<ref>PMID:18687679</ref>
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===NMR Structure of the N-terminal Coiled Coil Domain of the Andes Hantavirus Nucleocapsid Protein===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_18687679}}
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==About this Structure==
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[[2k48]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Andes_virus Andes virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K48 OCA].
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==See Also==
==See Also==
*[[Nucleoprotein|Nucleoprotein]]
*[[Nucleoprotein|Nucleoprotein]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:018687679</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Andes virus]]
[[Category: Andes virus]]
[[Category: Boudreaux, D M.]]
[[Category: Boudreaux, D M.]]

Revision as of 02:33, 29 September 2014

NMR Structure of the N-terminal Coiled Coil Domain of the Andes Hantavirus Nucleocapsid Protein

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