1lfo
From Proteopedia
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| - | [[Image:1lfo.gif|left|200px]] | + | [[Image:1lfo.gif|left|200px]] |
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| - | '''LIVER FATTY ACID BINDING PROTEIN-OLEATE COMPLEX''' | + | {{Structure |
| + | |PDB= 1lfo |SIZE=350|CAPTION= <scene name='initialview01'>1lfo</scene>, resolution 2.3Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene>, <scene name='pdbligand=BEO:BUTENOIC+ACID'>BEO</scene> and <scene name='pdbligand=UNX:UNKNOWN ATOM OR ION'>UNX</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''LIVER FATTY ACID BINDING PROTEIN-OLEATE COMPLEX''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LFO is a [ | + | 1LFO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LFO OCA]. |
==Reference== | ==Reference== | ||
| - | The crystal structure of the liver fatty acid-binding protein. A complex with two bound oleates., Thompson J, Winter N, Terwey D, Bratt J, Banaszak L, J Biol Chem. 1997 Mar 14;272(11):7140-50. PMID:[http:// | + | The crystal structure of the liver fatty acid-binding protein. A complex with two bound oleates., Thompson J, Winter N, Terwey D, Bratt J, Banaszak L, J Biol Chem. 1997 Mar 14;272(11):7140-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9054409 9054409] |
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: intracellular lipid transport protein]] | [[Category: intracellular lipid transport protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:30:23 2008'' |
Revision as of 10:30, 20 March 2008
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| , resolution 2.3Å | |||||||
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| Ligands: | , , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
LIVER FATTY ACID BINDING PROTEIN-OLEATE COMPLEX
Overview
The crystal structure of the recombinant form of rat liver fatty acid-binding protein was completed to 2.3 A and refined to an R factor of 19.0%. The structural solution was obtained by molecular replacement using superimposed polyalanine coordinates of six intracellular lipid-binding proteins as a search probe. The entire amino acid sequence of rat liver fatty acid-binding protein along with an amino-terminal formyl-methionine was modeled in the crystal structure. In addition, the crystal was obtained in the presence of oleic acid, and the initial electron density clearly showed two fatty acid molecules bound within a central cavity. The carboxylate of one fatty acid molecule interacts with arginine 122 and is shielded from free solvent. It has an overall bent conformation. The more solvent-exposed carboxylate of the other oleate is located near the helix-turn-helix that caps one end of the beta-barrel, while the acyl chain lies in the interior. The cavity contains both polar and nonpolar residues but also shows extensive hydrophobic character around the nonpolar atoms of the ligands. The primary and secondary oleate binding sites appear to be totally interdependent, mainly because favorable hydrophobic interactions form between both aliphatic chains.
About this Structure
1LFO is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
The crystal structure of the liver fatty acid-binding protein. A complex with two bound oleates., Thompson J, Winter N, Terwey D, Bratt J, Banaszak L, J Biol Chem. 1997 Mar 14;272(11):7140-50. PMID:9054409
Page seeded by OCA on Thu Mar 20 12:30:23 2008
