2j9n

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2j9n.png|left|200px]]
+
==Robotically harvested Trypsin complexed with Benzamidine containing polypeptide mediated crystal contacts==
 +
<StructureSection load='2j9n' size='340' side='right' caption='[[2j9n]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2j9n]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J9N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J9N FirstGlance]. <br>
 +
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IPH:PHENOL'>IPH</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
 +
<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
 +
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1aq7|1aq7]], [[1auj|1auj]], [[1az8|1az8]], [[1bju|1bju]], [[1bjv|1bjv]], [[1btp|1btp]], [[1btw|1btw]], [[1btx|1btx]], [[1bty|1bty]], [[1btz|1btz]], [[1c1n|1c1n]], [[1c1o|1c1o]], [[1c1p|1c1p]], [[1c1q|1c1q]], [[1c1r|1c1r]], [[1c1s|1c1s]], [[1c1t|1c1t]], [[1c2d|1c2d]], [[1c2e|1c2e]], [[1c2f|1c2f]], [[1c2g|1c2g]], [[1c2h|1c2h]], [[1c2i|1c2i]], [[1c2j|1c2j]], [[1c2k|1c2k]], [[1c2l|1c2l]], [[1c2m|1c2m]], [[1c5p|1c5p]], [[1c5q|1c5q]], [[1c5r|1c5r]], [[1c5s|1c5s]], [[1c5t|1c5t]], [[1c5u|1c5u]], [[1c5v|1c5v]], [[1c9t|1c9t]], [[1ce5|1ce5]], [[1cu7|1cu7]], [[1cu8|1cu8]], [[1cu9|1cu9]], [[1d6r|1d6r]], [[1eb2|1eb2]], [[1ejm|1ejm]], [[1ezx|1ezx]], [[1f0t|1f0t]], [[1f0u|1f0u]], [[1f2s|1f2s]], [[1g36|1g36]], [[1g3b|1g3b]], [[1g3c|1g3c]], [[1g3d|1g3d]], [[1g3e|1g3e]], [[1g9i|1g9i]], [[1gbt|1gbt]], [[1ghz|1ghz]], [[1gi0|1gi0]], [[1gi1|1gi1]], [[1gi2|1gi2]], [[1gi3|1gi3]], [[1gi4|1gi4]], [[1gi5|1gi5]], [[1gi6|1gi6]], [[1gj6|1gj6]], [[1hj9|1hj9]], [[1j8a|1j8a]], [[1jir|1jir]], [[1jrs|1jrs]], [[1jrt|1jrt]], [[1k1i|1k1i]], [[1k1j|1k1j]], [[1k1l|1k1l]], [[1k1m|1k1m]], [[1k1n|1k1n]], [[1k1o|1k1o]], [[1k1p|1k1p]], [[1lqe|1lqe]], [[1max|1max]], [[1may|1may]], [[1mts|1mts]], [[1mtu|1mtu]], [[1mtv|1mtv]], [[1mtw|1mtw]], [[1n6x|1n6x]], [[1n6y|1n6y]], [[1nc6|1nc6]], [[1ntp|1ntp]], [[1o2h|1o2h]], [[1o2i|1o2i]], [[1o2j|1o2j]], [[1o2k|1o2k]], [[1o2l|1o2l]], [[1o2m|1o2m]], [[1o2n|1o2n]], [[1o2o|1o2o]], [[1o2p|1o2p]], [[1o2q|1o2q]], [[1o2r|1o2r]], [[1o2s|1o2s]], [[1o2t|1o2t]], [[1o2u|1o2u]], [[1o2v|1o2v]], [[1o2w|1o2w]], [[1o2x|1o2x]], [[1o2y|1o2y]], [[1o2z|1o2z]], [[1o30|1o30]], [[1o31|1o31]], [[1o32|1o32]], [[1o33|1o33]], [[1o34|1o34]], [[1o35|1o35]], [[1o36|1o36]], [[1o37|1o37]], [[1o38|1o38]], [[1o39|1o39]], [[1o3a|1o3a]], [[1o3b|1o3b]], [[1o3c|1o3c]], [[1o3d|1o3d]], [[1o3e|1o3e]], [[1o3f|1o3f]], [[1o3g|1o3g]], [[1o3h|1o3h]], [[1o3i|1o3i]], [[1o3j|1o3j]], [[1o3k|1o3k]], [[1o3l|1o3l]], [[1o3m|1o3m]], [[1o3n|1o3n]], [[1o3o|1o3o]], [[1oph|1oph]], [[1ox1|1ox1]], [[1oyq|1oyq]], [[1p2i|1p2i]], [[1p2j|1p2j]], [[1p2k|1p2k]], [[1ppc|1ppc]], [[1ppe|1ppe]], [[1pph|1pph]], [[1qa0|1qa0]], [[1qb1|1qb1]], [[1qb6|1qb6]], [[1qb9|1qb9]], [[1qbn|1qbn]], [[1qbo|1qbo]], [[1qcp|1qcp]], [[1ql7|1ql7]], [[1ql8|1ql8]], [[1rxp|1rxp]], [[1s0q|1s0q]], [[1s0r|1s0r]], [[1sbw|1sbw]], [[1sfi|1sfi]], [[1smf|1smf]], [[1tab|1tab]], [[1taw|1taw]], [[1tgb|1tgb]], [[1tgc|1tgc]], [[1tgn|1tgn]], [[1tgs|1tgs]], [[1tgt|1tgt]], [[1tio|1tio]], [[1tld|1tld]], [[1tng|1tng]], [[1tnh|1tnh]], [[1tni|1tni]], [[1tnj|1tnj]], [[1tnk|1tnk]], [[1tnl|1tnl]], [[1tpa|1tpa]], [[1tpo|1tpo]], [[1tpp|1tpp]], [[1tps|1tps]], [[1tx7|1tx7]], [[1tx8|1tx8]], [[1tyn|1tyn]], [[1utn|1utn]], [[1uto|1uto]], [[1utp|1utp]], [[1utq|1utq]], [[1v2j|1v2j]], [[1v2k|1v2k]], [[1v2l|1v2l]], [[1v2m|1v2m]], [[1v2n|1v2n]], [[1v2o|1v2o]], [[1v2p|1v2p]], [[1v2q|1v2q]], [[1v2r|1v2r]], [[1v2s|1v2s]], [[1v2t|1v2t]], [[1v2u|1v2u]], [[1v2v|1v2v]], [[1v2w|1v2w]], [[1xuf|1xuf]], [[1xug|1xug]], [[1xuh|1xuh]], [[1xui|1xui]], [[1xuj|1xuj]], [[1xuk|1xuk]], [[1y3u|1y3u]], [[1y3v|1y3v]], [[1y3w|1y3w]], [[1y3x|1y3x]], [[1y3y|1y3y]], [[1y59|1y59]], [[1y5a|1y5a]], [[1y5b|1y5b]], [[1y5u|1y5u]], [[1yp9|1yp9]], [[1yyy|1yyy]], [[1zr0|1zr0]], [[1zzz|1zzz]], [[2a7h|2a7h]], [[2ah4|2ah4]], [[2ayw|2ayw]], [[2blv|2blv]], [[2blw|2blw]], [[2btc|2btc]], [[2by5|2by5]], [[2by6|2by6]], [[2by7|2by7]], [[2by8|2by8]], [[2by9|2by9]], [[2bya|2bya]], [[2bza|2bza]], [[2cmy|2cmy]], [[2fi3|2fi3]], [[2fi4|2fi4]], [[2fi5|2fi5]], [[2ftl|2ftl]], [[2ftm|2ftm]], [[2fx4|2fx4]], [[2fx6|2fx6]], [[2ptc|2ptc]], [[2ptn|2ptn]], [[2tga|2tga]], [[2tgd|2tgd]], [[2tgp|2tgp]], [[2tgt|2tgt]], [[2tio|2tio]], [[2tld|2tld]], [[2tpi|2tpi]], [[3btd|3btd]], [[3bte|3bte]], [[3btf|3btf]], [[3btg|3btg]], [[3bth|3bth]], [[3btk|3btk]], [[3btm|3btm]], [[3btq|3btq]], [[3btt|3btt]], [[3btw|3btw]], [[3ptb|3ptb]], [[3ptn|3ptn]], [[3tpi|3tpi]], [[4tpi|4tpi]], [[5ptp|5ptp]]</td></tr>
 +
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
 +
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j9n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2j9n RCSB], [http://www.ebi.ac.uk/pdbsum/2j9n PDBsum]</span></td></tr>
 +
<table>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j9/2j9n_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
High-throughput crystallography has reached a level of automation where complete computer-assisted robotic crystallization pipelines are capable of cocktail preparation, crystallization plate setup, and inspection and interpretation of results. While mounting of crystal pins, data collection and structure solution are highly automated, crystal harvesting and cryocooling remain formidable challenges towards full automation. To address the final frontier in achieving fully automated high-throughput crystallography, the prototype of an anthropomorphic six-axis universal micromanipulation robot (UMR) has been designed and tested; this UMR is capable of operator-assisted harvesting and cryoquenching of protein crystals as small as 10 microm from a variety of 96-well plates. The UMR is equipped with a versatile tool exchanger providing full operational flexibility. Trypsin crystals harvested and cryoquenched using the UMR have yielded a 1.5 A structure demonstrating the feasibility of robotic protein crystal harvesting.
-
{{STRUCTURE_2j9n| PDB=2j9n | SCENE= }}
+
Operator-assisted harvesting of protein crystals using a universal micromanipulation robot.,Viola R, Carman P, Walsh J, Miller E, Benning M, Frankel D, McPherson A, Cudney B, Rupp B J Appl Crystallogr. 2007 Jun 1;40(Pt 3):539-545. Epub 2007 May 15. PMID:19461845<ref>PMID:19461845</ref>
-
===Robotically harvested Trypsin complexed with Benzamidine containing polypeptide mediated crystal contacts===
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
{{ABSTRACT_PUBMED_19461845}}
+
-
 
+
-
==About this Structure==
+
-
[[2j9n]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J9N OCA].
+
==See Also==
==See Also==
*[[Trypsin|Trypsin]]
*[[Trypsin|Trypsin]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:019461845</ref><references group="xtra"/>
+
__TOC__
 +
</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Trypsin]]
[[Category: Trypsin]]

Revision as of 04:57, 29 September 2014

Robotically harvested Trypsin complexed with Benzamidine containing polypeptide mediated crystal contacts

2j9n, resolution 1.50Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2j9n"
Views
Personal tools
Navigation
Toolbox