2ye0
From Proteopedia
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| - | [[ | + | ==X-ray structure of the cyan fluorescent protein mTurquoise (K206A mutant)== |
| + | <StructureSection load='2ye0' size='340' side='right' caption='[[2ye0]], [[Resolution|resolution]] 1.47Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2ye0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YE0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YE0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SWG:2-[(4Z)-2-[(1R)-1-AMINO-2-HYDROXY-ETHYL]-4-(1H-INDOL-3-YLMETHYLIDENE)-5-OXO-IMIDAZOL-1-YL]ETHANOIC+ACID'>SWG</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2emn|2emn]], [[1jc0|1jc0]], [[1rm9|1rm9]], [[2fzu|2fzu]], [[1ema|1ema]], [[1rmo|1rmo]], [[1f09|1f09]], [[1yhg|1yhg]], [[1kp5|1kp5]], [[1q73|1q73]], [[2b3q|2b3q]], [[1z1p|1z1p]], [[1yhh|1yhh]], [[1rmm|1rmm]], [[1emm|1emm]], [[2fwq|2fwq]], [[2b3p|2b3p]], [[1emg|1emg]], [[1rmp|1rmp]], [[1emb|1emb]], [[2emd|2emd]], [[1q4e|1q4e]], [[1q4d|1q4d]], [[1kys|1kys]], [[1c4f|1c4f]], [[2wur|2wur]], [[1h6r|1h6r]], [[1q4a|1q4a]], [[1eml|1eml]], [[1w7t|1w7t]], [[1emk|1emk]], [[2emo|2emo]], [[2ydz|2ydz]], [[1q4b|1q4b]], [[2wsn|2wsn]], [[1w7u|1w7u]], [[1yjf|1yjf]], [[1q4c|1q4c]], [[1yfp|1yfp]], [[1emf|1emf]], [[1yj2|1yj2]], [[2ah8|2ah8]], [[2wso|2wso]], [[1yhi|1yhi]], [[2y0g|2y0g]], [[1eme|1eme]], [[1cv7|1cv7]], [[1gfl|1gfl]], [[1qyq|1qyq]], [[2ye1|2ye1]], [[1jbz|1jbz]], [[1kyr|1kyr]], [[2aha|2aha]], [[1rrx|1rrx]], [[1qxt|1qxt]], [[1myw|1myw]], [[1qy3|1qy3]], [[2yfp|2yfp]], [[1b9c|1b9c]], [[1w7s|1w7s]], [[1jby|1jby]], [[3ztf|3ztf]], [[1f0b|1f0b]], [[1jc1|1jc1]], [[1qyo|1qyo]], [[1bfp|1bfp]], [[1huy|1huy]], [[1emc|1emc]], [[1hcj|1hcj]], [[1z1q|1z1q]], [[1kyp|1kyp]], [[1qyf|1qyf]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ye0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ye0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ye0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ye0 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cyan variants of green fluorescent protein are widely used as donors in Forster resonance energy transfer experiments. The popular, but modestly bright, Enhanced Cyan Fluorescent Protein (ECFP) was sequentially improved into the brighter variants Super Cyan Fluorescent Protein 3A (SCFP3A) and mTurquoise, the latter exhibiting a high-fluorescence quantum yield and a long mono-exponential fluorescence lifetime. Here we combine X-ray crystallography and excited-state calculations to rationalize these stepwise improvements. The enhancement originates from stabilization of the seventh beta-strand and the strengthening of the sole chromophore-stabilizing hydrogen bond. The structural analysis highlighted one suboptimal internal residue, which was subjected to saturation mutagenesis combined with fluorescence lifetime-based screening. This resulted in mTurquoise2, a brighter variant with faster maturation, high photostability, longer mono-exponential lifetime and the highest quantum yield measured for a monomeric fluorescent protein. Together, these properties make mTurquoise2 the preferable cyan variant of green fluorescent protein for long-term imaging and as donor for Forster resonance energy transfer to a yellow fluorescent protein. | ||
| - | + | Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%.,Goedhart J, von Stetten D, Noirclerc-Savoye M, Lelimousin M, Joosen L, Hink MA, van Weeren L, Gadella TW Jr, Royant A Nat Commun. 2012 Mar 20;3:751. doi: 10.1038/ncomms1738. PMID:22434194<ref>PMID:22434194</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
*[[Green Fluorescent Protein|Green Fluorescent Protein]] | *[[Green Fluorescent Protein|Green Fluorescent Protein]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Aequorea victoria]] | [[Category: Aequorea victoria]] | ||
| - | [[Category: Gadella, T W.J | + | [[Category: Gadella, T W.J]] |
| - | [[Category: Goedhart, J | + | [[Category: Goedhart, J]] |
| - | [[Category: Hink, M A | + | [[Category: Hink, M A]] |
| - | [[Category: Joosen, L | + | [[Category: Joosen, L]] |
| - | [[Category: Lelimousin, M | + | [[Category: Lelimousin, M]] |
| - | [[Category: Noirclerc-Savoye, M | + | [[Category: Noirclerc-Savoye, M]] |
| - | [[Category: Royant, A | + | [[Category: Royant, A]] |
| - | [[Category: Stetten, D Von | + | [[Category: Stetten, D Von]] |
| - | [[Category: Weeren, L Van | + | [[Category: Weeren, L Van]] |
[[Category: Fluorescent protein]] | [[Category: Fluorescent protein]] | ||
[[Category: Fret donor]] | [[Category: Fret donor]] | ||
Revision as of 20:25, 24 December 2014
X-ray structure of the cyan fluorescent protein mTurquoise (K206A mutant)
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