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3fih

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[[Image:3fih.png|left|200px]]
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==Ternary complex-bound E.coli 70S ribosome. This entry consists of the 30S subunit, tRNAs and the ternary complex.==
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<StructureSection load='3fih' size='340' side='right' caption='[[3fih]], [[Resolution|resolution]] 6.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3fih]] is a 26 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FIH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FIH FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene><br>
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<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2i2u|2i2u]], [[2i2v|2i2v]], [[2j00|2j00]], [[1ob2|1ob2]], [[3fik|3fik]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fih OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fih RCSB], [http://www.ebi.ac.uk/pdbsum/3fih PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fi/3fih_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In translation, elongation factor Tu (EF-Tu) molecules deliver aminoacyl-tRNAs to the mRNA-programmed ribosome. The GTPase activity of EF-Tu is triggered by ribosome-induced conformational changes of the factor that play a pivotal role in the selection of the cognate aminoacyl-tRNAs. We present a 6.7-A cryo-electron microscopy map of the aminoacyl-tRNA x EF-Tu x GDP x kirromycin-bound Escherichia coli ribosome, together with an atomic model of the complex obtained through molecular dynamics flexible fitting. The model reveals the conformational changes in the conserved GTPase switch regions of EF-Tu that trigger hydrolysis of GTP, along with key interactions, including those between the sarcin-ricin loop and the P loop of EF-Tu, and between the effector loop of EF-Tu and a conserved region of the 16S rRNA. Our data suggest that GTP hydrolysis on EF-Tu is controlled through a hydrophobic gate mechanism.
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{{STRUCTURE_3fih| PDB=3fih | SCENE= }}
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Ribosome-induced changes in elongation factor Tu conformation control GTP hydrolysis.,Villa E, Sengupta J, Trabuco LG, LeBarron J, Baxter WT, Shaikh TR, Grassucci RA, Nissen P, Ehrenberg M, Schulten K, Frank J Proc Natl Acad Sci U S A. 2009 Jan 27;106(4):1063-8. Epub 2009 Jan 2. PMID:19122150<ref>PMID:19122150</ref>
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===Ternary complex-bound E.coli 70S ribosome. This entry consists of the 30S subunit, tRNAs and the ternary complex.===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19122150}}
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==About this Structure==
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[[3fih]] is a 26 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FIH OCA].
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==See Also==
==See Also==
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*[[Ribosomal protein S10|Ribosomal protein S10]]
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*[[Elongation factor|Elongation factor]]
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*[[Ribosomal protein S11|Ribosomal protein S11]]
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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*[[Ribosomal protein S12|Ribosomal protein S12]]
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== References ==
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*[[Ribosomal protein S13|Ribosomal protein S13]]
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<references/>
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*[[Ribosomal protein S14|Ribosomal protein S14]]
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__TOC__
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*[[Ribosomal protein S15|Ribosomal protein S15]]
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</StructureSection>
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*[[Ribosomal protein S16|Ribosomal protein S16]]
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*[[Ribosomal protein S17|Ribosomal protein S17]]
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*[[Ribosomal protein S18|Ribosomal protein S18]]
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*[[Ribosomal protein S19|Ribosomal protein S19]]
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*[[Ribosomal protein S2|Ribosomal protein S2]]
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*[[Ribosomal protein S20|Ribosomal protein S20]]
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*[[Ribosomal protein S21|Ribosomal protein S21]]
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*[[Ribosomal protein S3|Ribosomal protein S3]]
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*[[Ribosomal protein S4|Ribosomal protein S4]]
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*[[Ribosomal protein S5|Ribosomal protein S5]]
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*[[Ribosomal protein S6|Ribosomal protein S6]]
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*[[Ribosomal protein S7|Ribosomal protein S7]]
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*[[Ribosomal protein S8|Ribosomal protein S8]]
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*[[Ribosomal protein S9|Ribosomal protein S9]]
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==Reference==
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<ref group="xtra">PMID:019122150</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Baxter, W T.]]
[[Category: Baxter, W T.]]

Revision as of 13:40, 29 September 2014

Ternary complex-bound E.coli 70S ribosome. This entry consists of the 30S subunit, tRNAs and the ternary complex.

3fih, resolution 6.70Å

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