3mt5

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[[Image:3mt5.png|left|200px]]
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==Crystal Structure of the Human BK Gating Apparatus==
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<StructureSection load='3mt5' size='340' side='right' caption='[[3mt5]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3mt5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MT5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MT5 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KCNMA1, RP11-443A13.1-004 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mt5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mt5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mt5 RCSB], [http://www.ebi.ac.uk/pdbsum/3mt5 PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mt/3mt5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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High-conductance voltage- and Ca2+-activated K+ (BK) channels encode negative feedback regulation of membrane voltage and Ca2+ signaling, playing a central role in numerous physiological processes. We determined the x-ray structure of the human BK Ca2+ gating apparatus at a resolution of 3.0 angstroms and deduced its tetrameric assembly by solving a 6 angstrom resolution structure of a Na+-activated homolog. Two tandem C-terminal regulator of K+ conductance (RCK) domains from each of four channel subunits form a 350-kilodalton gating ring at the intracellular membrane surface. A sequence of aspartic amino acids that is known as the Ca2+ bowl, and is located within the second of the tandem RCK domains, creates four Ca2+ binding sites on the outer perimeter of the gating ring at the "assembly interface" between RCK domains. Functionally important mutations cluster near the Ca2+ bowl, near the "flexible interface" between RCK domains, and on the surface of the gating ring that faces the voltage sensors. The structure suggests that the Ca2+ gating ring, in addition to regulating the pore directly, may also modulate the voltage sensor.
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{{STRUCTURE_3mt5| PDB=3mt5 | SCENE= }}
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Structure of the human BK channel Ca2+-activation apparatus at 3.0 A resolution.,Yuan P, Leonetti MD, Pico AR, Hsiung Y, MacKinnon R Science. 2010 Jul 9;329(5988):182-6. Epub 2010 May 27. PMID:20508092<ref>PMID:20508092</ref>
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===Crystal Structure of the Human BK Gating Apparatus===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_20508092}}
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==About this Structure==
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[[3mt5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MT5 OCA].
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==See Also==
==See Also==
*[[Potassium Channel|Potassium Channel]]
*[[Potassium Channel|Potassium Channel]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020508092</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: MacKinnon, R.]]
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[[Category: MacKinnon, R]]
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[[Category: Yuan, P.]]
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[[Category: Yuan, P]]
[[Category: Membrane protein]]
[[Category: Membrane protein]]
[[Category: Potassium channel]]
[[Category: Potassium channel]]
[[Category: Transport protein]]
[[Category: Transport protein]]

Revision as of 10:03, 9 December 2014

Crystal Structure of the Human BK Gating Apparatus

3mt5, resolution 3.00Å

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