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2j00
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| - | [[ | + | ==STRUCTURE OF THE THERMUS THERMOPHILUS 70S RIBOSOME COMPLEXED WITH MRNA, TRNA AND PAROMOMYCIN (PART 1 OF 4). THIS FILE CONTAINS THE 30S SUBUNIT, MRNA, A-, P- AND E-SITE TRNAS AND PAROMOMYCIN FOR MOLECULE I.== |
| + | <StructureSection load='2j00' size='340' side='right' caption='[[2j00]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2j00]] is a 25 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J00 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J00 FirstGlance]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br> | ||
| + | <tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene></td></tr> | ||
| + | <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2j01|2j01]], [[2j02|2j02]], [[2j03|2j03]], [[1fjg|1fjg]], [[1gix|1gix]], [[1i94|1i94]], [[1i95|1i95]], [[1i96|1i96]], [[1i97|1i97]], [[1ibk|1ibk]], [[1ibl|1ibl]], [[1ibm|1ibm]], [[1j5e|1j5e]], [[1jgo|1jgo]], [[1jgp|1jgp]], [[1jgq|1jgq]], [[1l1u|1l1u]], [[1n32|1n32]], [[1n33|1n33]], [[1n34|1n34]], [[1n36|1n36]], [[1pns|1pns]], [[1pnx|1pnx]], [[1xmo|1xmo]], [[1xmq|1xmq]], [[1xnq|1xnq]], [[1xnr|1xnr]], [[1yl4|1yl4]], [[2b64|2b64]], [[2b9m|2b9m]], [[2b9o|2b9o]], [[2f4v|2f4v]]</td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j00 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2j00 RCSB], [http://www.ebi.ac.uk/pdbsum/2j00 PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j0/2j00_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The crystal structure of the bacterial 70S ribosome refined to 2.8 angstrom resolution reveals atomic details of its interactions with messenger RNA (mRNA) and transfer RNA (tRNA). A metal ion stabilizes a kink in the mRNA that demarcates the boundary between A and P sites, which is potentially important to prevent slippage of mRNA. Metal ions also stabilize the intersubunit interface. The interactions of E-site tRNA with the 50S subunit have both similarities and differences compared to those in the archaeal ribosome. The structure also rationalizes much biochemical and genetic data on translation. | ||
| - | + | Structure of the 70S ribosome complexed with mRNA and tRNA.,Selmer M, Dunham CM, Murphy FV 4th, Weixlbaumer A, Petry S, Kelley AC, Weir JR, Ramakrishnan V Science. 2006 Sep 29;313(5795):1935-42. Epub 2006 Sep 7. PMID:16959973<ref>PMID:16959973</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
| - | *[[Ribosomal protein S10|Ribosomal protein S10]] | ||
| - | *[[Ribosomal protein S11|Ribosomal protein S11]] | ||
| - | *[[Ribosomal protein S12|Ribosomal protein S12]] | ||
| - | *[[Ribosomal protein S13|Ribosomal protein S13]] | ||
| - | *[[Ribosomal protein S14|Ribosomal protein S14]] | ||
| - | *[[Ribosomal protein S15|Ribosomal protein S15]] | ||
| - | *[[Ribosomal protein S16|Ribosomal protein S16]] | ||
| - | *[[Ribosomal protein S17|Ribosomal protein S17]] | ||
| - | *[[Ribosomal protein S18|Ribosomal protein S18]] | ||
| - | *[[Ribosomal protein S19|Ribosomal protein S19]] | ||
| - | *[[Ribosomal protein S2|Ribosomal protein S2]] | ||
| - | *[[Ribosomal protein S20|Ribosomal protein S20]] | ||
| - | *[[Ribosomal protein S3|Ribosomal protein S3]] | ||
| - | *[[Ribosomal protein S4|Ribosomal protein S4]] | ||
| - | *[[Ribosomal protein S5|Ribosomal protein S5]] | ||
| - | *[[Ribosomal protein S6|Ribosomal protein S6]] | ||
| - | *[[Ribosomal protein S7|Ribosomal protein S7]] | ||
| - | *[[Ribosomal protein S8|Ribosomal protein S8]] | ||
| - | *[[Ribosomal protein S9|Ribosomal protein S9]] | ||
*[[Ribosomal protein THX|Ribosomal protein THX]] | *[[Ribosomal protein THX|Ribosomal protein THX]] | ||
| - | *[[Ribosome|Ribosome]] | + | *[[Ribosome 3D structures|Ribosome 3D structures]] |
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
Revision as of 02:57, 29 September 2014
STRUCTURE OF THE THERMUS THERMOPHILUS 70S RIBOSOME COMPLEXED WITH MRNA, TRNA AND PAROMOMYCIN (PART 1 OF 4). THIS FILE CONTAINS THE 30S SUBUNIT, MRNA, A-, P- AND E-SITE TRNAS AND PAROMOMYCIN FOR MOLECULE I.
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