1lva
From Proteopedia
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| - | [[Image:1lva.gif|left|200px]] | + | [[Image:1lva.gif|left|200px]] |
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| - | '''Crystal structure of a C-terminal fragment of Moorella thermoacetica elongation factor SelB''' | + | {{Structure |
| + | |PDB= 1lva |SIZE=350|CAPTION= <scene name='initialview01'>1lva</scene>, resolution 2.12Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=Y1:YTTRIUM+ION'>Y1</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= SelB(amino acids 370-634) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1525 Moorella thermoacetica]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of a C-terminal fragment of Moorella thermoacetica elongation factor SelB''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LVA is a [ | + | 1LVA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVA OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of an mRNA-binding fragment of Moorella thermoacetica elongation factor SelB., Selmer M, Su XD, EMBO J. 2002 Aug 1;21(15):4145-53. PMID:[http:// | + | Crystal structure of an mRNA-binding fragment of Moorella thermoacetica elongation factor SelB., Selmer M, Su XD, EMBO J. 2002 Aug 1;21(15):4145-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12145214 12145214] |
[[Category: Moorella thermoacetica]] | [[Category: Moorella thermoacetica]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: winged-helix]] | [[Category: winged-helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:35:37 2008'' |
Revision as of 10:35, 20 March 2008
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| , resolution 2.12Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | SelB(amino acids 370-634) (Moorella thermoacetica) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a C-terminal fragment of Moorella thermoacetica elongation factor SelB
Overview
SelB is an elongation factor needed for the co-translational incorporation of selenocysteine. Selenocysteine is coded by a UGA stop codon in combination with a specific downstream mRNA hairpin. In bacteria, the C-terminal part of SelB recognizes this hairpin, while the N-terminal part binds GTP and tRNA in analogy with elongation factor Tu (EF-Tu). We present the crystal structure of a C-terminal fragment of SelB (SelB-C) from Moorella thermoacetica at 2.12 A resolution, solved by a combination of selenium and yttrium multiwavelength anomalous dispersion. This 264 amino acid fragment contains the entire C-terminal extension beginning after the EF-Tu-homologous domains. SelB-C consists of four similar winged-helix domains arranged into the shape of an L. This is the first example of winged-helix domains involved in RNA binding. The location of conserved basic amino acids, together with data from the literature, define the position of the mRNA-binding site. Steric requirements indicate that a conformational change may occur upon ribosome interaction. Structural observations and data in the literature suggest that this change happens upon mRNA binding.
About this Structure
1LVA is a Single protein structure of sequence from Moorella thermoacetica. Full crystallographic information is available from OCA.
Reference
Crystal structure of an mRNA-binding fragment of Moorella thermoacetica elongation factor SelB., Selmer M, Su XD, EMBO J. 2002 Aug 1;21(15):4145-53. PMID:12145214
Page seeded by OCA on Thu Mar 20 12:35:37 2008
