1ly1

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[[Image:1ly1.jpg|left|200px]]<br /><applet load="1ly1" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1ly1.jpg|left|200px]]
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caption="1ly1, resolution 2.00&Aring;" />
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'''Structure and Mechanism of T4 Polynucleotide Kinase'''<br />
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{{Structure
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|PDB= 1ly1 |SIZE=350|CAPTION= <scene name='initialview01'>1ly1</scene>, resolution 2.00&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Polynucleotide_5'-hydroxy-kinase Polynucleotide 5'-hydroxy-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.78 2.7.1.78]
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|GENE= PSET ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Bacteriophage T4])
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}}
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'''Structure and Mechanism of T4 Polynucleotide Kinase'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1LY1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Polynucleotide_5'-hydroxy-kinase Polynucleotide 5'-hydroxy-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.78 2.7.1.78] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LY1 OCA].
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1LY1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LY1 OCA].
==Reference==
==Reference==
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Structure and mechanism of T4 polynucleotide kinase: an RNA repair enzyme., Wang LK, Lima CD, Shuman S, EMBO J. 2002 Jul 15;21(14):3873-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12110598 12110598]
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Structure and mechanism of T4 polynucleotide kinase: an RNA repair enzyme., Wang LK, Lima CD, Shuman S, EMBO J. 2002 Jul 15;21(14):3873-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12110598 12110598]
[[Category: Bacteriophage t4]]
[[Category: Bacteriophage t4]]
[[Category: Polynucleotide 5'-hydroxy-kinase]]
[[Category: Polynucleotide 5'-hydroxy-kinase]]
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[[Category: t4]]
[[Category: t4]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:49:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:36:33 2008''

Revision as of 10:36, 20 March 2008

Image:1ly1.jpg


PDB ID 1ly1

Drag the structure with the mouse to rotate
, resolution 2.00Å
Ligands:
Gene: PSET (Bacteriophage T4)
Activity: Polynucleotide 5'-hydroxy-kinase, with EC number 2.7.1.78
Coordinates: save as pdb, mmCIF, xml



Structure and Mechanism of T4 Polynucleotide Kinase


Overview

T4 polynucleotide kinase (Pnk), in addition to being an invaluable research tool, exemplifies a family of bifunctional enzymes with 5'-kinase and 3'-phosphatase activities that play key roles in RNA and DNA repair. T4 Pnk is a homotetramer composed of a C-terminal phosphatase domain and an N-terminal kinase domain. The 2.0 A crystal structure of the isolated kinase domain highlights a tunnel-like active site through the heart of the enzyme, with an entrance on the 5' OH acceptor side that can accommodate a single-stranded polynucleotide. The active site is composed of essential side chains that coordinate the beta phosphate of the NTP donor and the 3' phosphate of the 5' OH acceptor, plus a putative general acid that activates the 5' OH. The structure rationalizes the different specificities of T4 and eukaryotic Pnk and suggests a model for the assembly of the tetramer.

About this Structure

1LY1 is a Single protein structure of sequence from Bacteriophage t4. Full crystallographic information is available from OCA.

Reference

Structure and mechanism of T4 polynucleotide kinase: an RNA repair enzyme., Wang LK, Lima CD, Shuman S, EMBO J. 2002 Jul 15;21(14):3873-80. PMID:12110598

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