1ly2
From Proteopedia
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| - | [[Image:1ly2.jpg|left|200px]] | + | [[Image:1ly2.jpg|left|200px]] |
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| - | '''Crystal structure of unliganded human CD21 SCR1-SCR2 (Complement receptor type 2)''' | + | {{Structure |
| + | |PDB= 1ly2 |SIZE=350|CAPTION= <scene name='initialview01'>1ly2</scene>, resolution 1.80Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= CR2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of unliganded human CD21 SCR1-SCR2 (Complement receptor type 2)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LY2 is a [ | + | 1LY2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LY2 OCA]. |
==Reference== | ==Reference== | ||
| - | The crystal structure of human CD21: Implications for Epstein-Barr virus and C3d binding., Prota AE, Sage DR, Stehle T, Fingeroth JD, Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10641-6. Epub 2002 Jul 16. PMID:[http:// | + | The crystal structure of human CD21: Implications for Epstein-Barr virus and C3d binding., Prota AE, Sage DR, Stehle T, Fingeroth JD, Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10641-6. Epub 2002 Jul 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12122212 12122212] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: complement receptor; epstein barr virus; regulator of complement activation; short consensus repeat; viral receptor; complement control protein]] | [[Category: complement receptor; epstein barr virus; regulator of complement activation; short consensus repeat; viral receptor; complement control protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:36:34 2008'' |
Revision as of 10:36, 20 March 2008
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| , resolution 1.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | CR2 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of unliganded human CD21 SCR1-SCR2 (Complement receptor type 2)
Contents |
Overview
Human complement receptor type 2 (CD21) is the cellular receptor for Epstein-Barr virus (EBV), a human tumor virus. The N-terminal two short consensus repeats (SCR1-SCR2) of the receptor interact with the EBV glycoprotein gp350/220 and also with the natural CD21 ligand C3d. Here we present the crystal structure of the CD21 SCR1-SCR2 fragment in the absence of ligand and demonstrate that it is able to bind EBV. Based on a functional analysis of wild-type and mutant CD21 and molecular modeling, we identify a likely region for EBV attachment and demonstrate that this region is not involved in the interaction with C3d. A comparison with the previously determined structure of CD21 SCR1-SCR2 in complex with C3d shows that, in both cases, CD21 assumes compact V-shaped conformations. However, our analysis reveals a surprising degree of flexibility at the SCR1-SCR2 interface, suggesting interactions between the two domains are not specific. We present evidence that the V-shaped conformation is induced by deglycosylation of the protein, and that physiologic glycosylation of CD21 would result in a more extended conformation, perhaps with additional epitopes for C3d binding.
Disease
Known diseases associated with this structure: Systemic lupus erythematosus, susceptibility to, 9 OMIM:[120650]
About this Structure
1LY2 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The crystal structure of human CD21: Implications for Epstein-Barr virus and C3d binding., Prota AE, Sage DR, Stehle T, Fingeroth JD, Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10641-6. Epub 2002 Jul 16. PMID:12122212
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