1m1f

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[[Image:1m1f.jpg|left|200px]]<br /><applet load="1m1f" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1m1f.jpg|left|200px]]
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caption="1m1f, resolution 1.4&Aring;" />
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'''Kid toxin protein from E.coli plasmid R1'''<br />
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{{Structure
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|PDB= 1m1f |SIZE=350|CAPTION= <scene name='initialview01'>1m1f</scene>, resolution 1.4&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Kid toxin protein from E.coli plasmid R1'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1M1F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M1F OCA].
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1M1F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M1F OCA].
==Reference==
==Reference==
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Structural and functional analysis of the kid toxin protein from E. coli plasmid R1., Hargreaves D, Santos-Sierra S, Giraldo R, Sabariegos-Jareno R, de la Cueva-Mendez G, Boelens R, Diaz-Orejas R, Rafferty JB, Structure. 2002 Oct;10(10):1425-33. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12377128 12377128]
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Structural and functional analysis of the kid toxin protein from E. coli plasmid R1., Hargreaves D, Santos-Sierra S, Giraldo R, Sabariegos-Jareno R, de la Cueva-Mendez G, Boelens R, Diaz-Orejas R, Rafferty JB, Structure. 2002 Oct;10(10):1425-33. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12377128 12377128]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: toxin-antitoxin]]
[[Category: toxin-antitoxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:50:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:37:41 2008''

Revision as of 10:37, 20 March 2008

Image:1m1f.jpg


PDB ID 1m1f

Drag the structure with the mouse to rotate
, resolution 1.4Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



Kid toxin protein from E.coli plasmid R1


Overview

We have determined the structure of Kid toxin protein from E. coli plasmid R1 involved in stable plasmid inheritance by postsegregational killing of plasmid-less daughter cells. Kid forms a two-component system with its antagonist, Kis antitoxin. Our 1.4 A crystal structure of Kid reveals a 2-fold symmetric dimer that closely resembles the DNA gyrase-inhibitory toxin protein CcdB from E. coli F plasmid despite the lack of any notable sequence similarity. Analysis of nontoxic mutants of Kid suggests a target interaction interface associated with toxicity that is in marked contrast to that proposed for CcdB. A possible region for interaction of Kid with the antitoxin is proposed that overlaps with the target binding site and may explain the mode of antitoxin action.

About this Structure

1M1F is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural and functional analysis of the kid toxin protein from E. coli plasmid R1., Hargreaves D, Santos-Sierra S, Giraldo R, Sabariegos-Jareno R, de la Cueva-Mendez G, Boelens R, Diaz-Orejas R, Rafferty JB, Structure. 2002 Oct;10(10):1425-33. PMID:12377128

Page seeded by OCA on Thu Mar 20 12:37:41 2008

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