3srp
From Proteopedia
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| - | [[ | + | ==Structure of Rivax: A Human Ricin Vaccine== |
| + | <StructureSection load='3srp' size='340' side='right' caption='[[3srp]], [[Resolution|resolution]] 2.14Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3srp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ricinus_communis Ricinus communis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SRP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SRP FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3bjg|3bjg]], [[3lc9|3lc9]], [[3mk9|3mk9]]</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3srp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3srp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3srp RCSB], [http://www.ebi.ac.uk/pdbsum/3srp PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | RiVax is a recombinant protein that is currently under clinical development as part of a human vaccine to protect against ricin poisoning. RiVax includes ricin A-chain (RTA) residues 1-267 with two intentional amino-acid substitutions, V76M and Y80A, aimed at reducing toxicity. Here, the crystal structure of RiVax was solved to 2.1 A resolution and it was shown that it is superposable with that of the ricin toxin A-chain from Ricinus communis with a root-mean-square deviation of 0.6 A over 258 C(alpha) atoms. The RiVax structure is also compared with the recently determined structure of another potential ricin-vaccine immunogen, RTA 1-33/44-198 R48C/T77C. Finally, the locations and solvent-exposure of two toxin-neutralizing B-cell epitopes were examined and it was found that these epitopes are within or near regions predicted to be involved in catalysis. The results demonstrate the composition of the RiVax clinical material and will guide ongoing protein-engineering strategies to develop improved immunogens. | ||
| - | + | Structure of RiVax: a recombinant ricin vaccine.,Legler PM, Brey RN, Smallshaw JE, Vitetta ES, Millard CB Acta Crystallogr D Biol Crystallogr. 2011 Sep;67(Pt 9):826-30. doi:, 10.1107/S0907444911026771. Epub 2011 Aug 9. PMID:21904036<ref>PMID:21904036</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
*[[Ricin|Ricin]] | *[[Ricin|Ricin]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Ricinus communis]] | [[Category: Ricinus communis]] | ||
[[Category: RRNA N-glycosylase]] | [[Category: RRNA N-glycosylase]] | ||
| - | [[Category: Legler, P M | + | [[Category: Legler, P M]] |
| - | [[Category: Millard, C B | + | [[Category: Millard, C B]] |
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Immunogen]] | [[Category: Immunogen]] | ||
[[Category: Ribosome inactivating protein]] | [[Category: Ribosome inactivating protein]] | ||
Revision as of 13:01, 9 December 2014
Structure of Rivax: A Human Ricin Vaccine
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