3spu
From Proteopedia
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| - | [[ | + | ==apo NDM-1 Crystal Structure== |
| + | <StructureSection load='3spu' size='340' side='right' caption='[[3spu]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3spu]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SPU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SPU FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">blaNDM-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 Klebsiella pneumoniae])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3spu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3spu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3spu RCSB], [http://www.ebi.ac.uk/pdbsum/3spu PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | beta-Lactams are the most commonly prescribed class of antibiotics and have had an enormous impact on human health. Thus, it is disquieting that an enzyme called New Delhi metallo-beta-lactamase-1 (NDM-1) can confer Enterobacteriaceae with nearly complete resistance to all beta-lactam antibiotics including the carbapenams. We have determined the crystal structure of Klebsiella pneumoniae apo-NDM-1 to 2.1-A resolution. From the structure, we see that NDM-1 has an expansive active site with a unique electrostatic profile, which we propose leads to a broader substrate specificity. In addition, NDM-1 undergoes important conformational changes upon substrate binding. These changes have not been previously observed in metallo-beta-lactamase enzymes and may have a direct influence on substrate recognition and catalysis. | ||
| - | + | Crystal structure of New Delhi metallo-beta-lactamase reveals molecular basis for antibiotic resistance.,King D, Strynadka N Protein Sci. 2011 Sep;20(9):1484-91. doi: 10.1002/pro.697. Epub 2011 Aug, 2. PMID:21774017<ref>PMID:21774017</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
*[[Beta-lactamase|Beta-lactamase]] | *[[Beta-lactamase|Beta-lactamase]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Beta-lactamase]] | [[Category: Beta-lactamase]] | ||
[[Category: Klebsiella pneumoniae]] | [[Category: Klebsiella pneumoniae]] | ||
| - | [[Category: King, D T | + | [[Category: King, D T]] |
| - | [[Category: Strynadka, N C.J | + | [[Category: Strynadka, N C.J]] |
[[Category: Alpha beta/beta alpha sandwich]] | [[Category: Alpha beta/beta alpha sandwich]] | ||
[[Category: Beta lactam antibiotic]] | [[Category: Beta lactam antibiotic]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Periplasmic space]] | [[Category: Periplasmic space]] | ||
Revision as of 13:22, 9 December 2014
apo NDM-1 Crystal Structure
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