1m50
From Proteopedia
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| - | [[Image:1m50.gif|left|200px]] | + | [[Image:1m50.gif|left|200px]] |
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| - | '''Crystal Structure Of The Bacteriochlorophyll A Protein From Chlorobium Tepidum''' | + | {{Structure |
| + | |PDB= 1m50 |SIZE=350|CAPTION= <scene name='initialview01'>1m50</scene>, resolution 2.20Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene> and <scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL A'>BCL</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure Of The Bacteriochlorophyll A Protein From Chlorobium Tepidum''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1M50 is a [ | + | 1M50 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Chlorobaculum_tepidum Chlorobaculum tepidum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M50 OCA]. |
==Reference== | ==Reference== | ||
| - | The structure of the FMO protein from Chlorobium tepidum at 2.2 A resolution., Camara-Artigas A, Blankenship RE, Allen JP, Photosynth Res. 2003;75(1):49-55. PMID:[http:// | + | The structure of the FMO protein from Chlorobium tepidum at 2.2 A resolution., Camara-Artigas A, Blankenship RE, Allen JP, Photosynth Res. 2003;75(1):49-55. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16245093 16245093] |
[[Category: Chlorobaculum tepidum]] | [[Category: Chlorobaculum tepidum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: beta sheet]] | [[Category: beta sheet]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:38:56 2008'' |
Revision as of 10:38, 20 March 2008
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| , resolution 2.20Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure Of The Bacteriochlorophyll A Protein From Chlorobium Tepidum
Overview
The bacteriochlorophyll protein, or FMO protein, from Chlorobium tepidum, which serves as a light-harvesting complex and directs light energy from the chlorosomes attached to the cell membrane to the reaction center has been crystallized in a new space group. The crystals belong to the cubic space group P4(3)32 and the structure has been refined to a resolution 2.2 A with a R factor of 19.7%. The electron density maps show that the structure is composed of two beta sheets that surround seven bacteriochlorophylls as previously reported (Li et al. (1997) J Mol Biol 271: 456-471). The availability of the new data allows a more accurate refinement of the pigment-protein complex including identification of bound solvent molecules. Several structural differences probably contribute to the observed spectroscopic differences between the FMO proteins from Cb. tepidum and Prosthecochloris aestuarii, including differences in the planarity of corresponding tetrapyrroles. A citrate molecule is found on the surface of each protein subunit of the trimer from Cb. tepidum. However, the citrate molecule is over 15 A from any bacteriochlorophyll. The presence of the citrate probably does not contribute to the function of the protein although it does contribute to the crystallization as it interacts with a crystallographically related trimer. Among the 236 water molecules found in the protein are four that appear to play a special role in the properties of bacteriochlorophyll 2, as this tetrapyrrole is coordinated by one of these water molecules and the waters form a hydrogen-bonded network that leads to the surface of the protein.
About this Structure
1M50 is a Single protein structure of sequence from Chlorobaculum tepidum. Full crystallographic information is available from OCA.
Reference
The structure of the FMO protein from Chlorobium tepidum at 2.2 A resolution., Camara-Artigas A, Blankenship RE, Allen JP, Photosynth Res. 2003;75(1):49-55. PMID:16245093
Page seeded by OCA on Thu Mar 20 12:38:56 2008
