1m60
From Proteopedia
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| - | [[Image:1m60.gif|left|200px]] | + | [[Image:1m60.gif|left|200px]] |
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| - | '''Solution Structure of Zinc-substituted cytochrome c''' | + | {{Structure |
| + | |PDB= 1m60 |SIZE=350|CAPTION= <scene name='initialview01'>1m60</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=HES:ZINC SUBSTITUTED HEME C'>HES</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Solution Structure of Zinc-substituted cytochrome c''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1M60 is a [ | + | 1M60 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M60 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural analysis of zinc-substituted cytochrome c., Qian C, Yao Y, Tong Y, Wang J, Tang W, J Biol Inorg Chem. 2003 Apr;8(4):394-400. Epub 2002 Dec 14. PMID:[http:// | + | Structural analysis of zinc-substituted cytochrome c., Qian C, Yao Y, Tong Y, Wang J, Tang W, J Biol Inorg Chem. 2003 Apr;8(4):394-400. Epub 2002 Dec 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12761660 12761660] |
[[Category: Equus caballus]] | [[Category: Equus caballus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: six-coordinated zinc cyt c]] | [[Category: six-coordinated zinc cyt c]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:39:19 2008'' |
Revision as of 10:39, 20 March 2008
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Solution Structure of Zinc-substituted cytochrome c
Overview
Zinc-substituted cytochrome c has been widely used in studies of protein-protein interactions and photo-induced electron transfer reactions between proteins. However, the coordination geometry of zinc in zinc-substituted cyt c has not yet been determined; two different opinions about the coordination have been reached. Here the solution structures of zinc-substituted cytochrome c that might be five-coordinated and six-coordinated have been refined separately by using (1)H NMR spectroscopy, and the zinc coordination geometry was determined just by NOE distance constraints. Structural analysis of the energy-minimized average solution structures of both the pentacoordinated and hexacoordinated geometries indicate that that zinc in zinc-substituted cyt c should be bound to both His18 and Met80, which means that the zinc is six-coordinated. RMSD values of the family of 25 six-coordinated structures from the average structure are 0.66+/-0.13 A and 1.09+/-0.16 A for the backbone and all heavy atoms, respectively. A statistical analysis of the structure indicates its satisfactory quality. Comparison of the solution structure of the six-coordinated energy-minimized average structure of zinc-substituted cytochrome c with the solution structure of reduced cytochrome c reveals that for the overall folding the secondary structure elements are very close. The availability of the structure provides for a better understanding of the protein-protein complex and for electron transfer processes between Zn cyt c and other metalloproteins.
About this Structure
1M60 is a Single protein structure of sequence from Equus caballus. Full crystallographic information is available from OCA.
Reference
Structural analysis of zinc-substituted cytochrome c., Qian C, Yao Y, Tong Y, Wang J, Tang W, J Biol Inorg Chem. 2003 Apr;8(4):394-400. Epub 2002 Dec 14. PMID:12761660
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