This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1ma8
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1ma8.gif|left|200px]] | + | [[Image:1ma8.gif|left|200px]] |
| - | + | ||
| - | '''A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine''' | + | {{Structure |
| + | |PDB= 1ma8 |SIZE=350|CAPTION= <scene name='initialview01'>1ma8</scene>, resolution 1.3Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1MA8 is a [ | + | 1MA8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MA8 OCA]. |
==Reference== | ==Reference== | ||
| - | Covalent incorporation of selenium into oligonucleotides for X-ray crystal structure determination via MAD: proof of principle. Multiwavelength anomalous dispersion., Teplova M, Wilds CJ, Wawrzak Z, Tereshko V, Du Q, Carrasco N, Huang Z, Egli M, Biochimie. 2002 Sep;84(9):849-58. PMID:[http:// | + | Covalent incorporation of selenium into oligonucleotides for X-ray crystal structure determination via MAD: proof of principle. Multiwavelength anomalous dispersion., Teplova M, Wilds CJ, Wawrzak Z, Tereshko V, Du Q, Carrasco N, Huang Z, Egli M, Biochimie. 2002 Sep;84(9):849-58. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12458077 12458077] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Carrasco, N.]] | [[Category: Carrasco, N.]] | ||
| Line 22: | Line 31: | ||
[[Category: 2'-methylseleno-uridine]] | [[Category: 2'-methylseleno-uridine]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:40:57 2008'' |
Revision as of 10:40, 20 March 2008
| |||||||
| , resolution 1.3Å | |||||||
|---|---|---|---|---|---|---|---|
| Coordinates: | save as pdb, mmCIF, xml | ||||||
A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine
Overview
Selenium was incorporated into an oligodeoxynucleotide in the form of 2'-methylseleno-uridine (U(Se)). The X-ray crystal structure of the duplex left open bracket d(GCGTA)U(Se)d(ACGC) right open bracket (2) was determined by the multiwavelength anomalous dispersion (MAD) technique and refined to a resolution of 1.3 A, demonstrating that selenium can selectively substitute oxygen in DNA and that the resulting compounds are chemically stable. Since derivatization at the 2'-alpha-position with selenium does not affect the preference of the sugar for the C3'-endo conformation, this strategy is suitable for incorporating selenium into RNA. The availability of selenium-containing nucleic acids for crystallographic phasing offers an attractive alternative to the commonly used halogenated pyrimidines.
About this Structure
1MA8 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Covalent incorporation of selenium into oligonucleotides for X-ray crystal structure determination via MAD: proof of principle. Multiwavelength anomalous dispersion., Teplova M, Wilds CJ, Wawrzak Z, Tereshko V, Du Q, Carrasco N, Huang Z, Egli M, Biochimie. 2002 Sep;84(9):849-58. PMID:12458077
Page seeded by OCA on Thu Mar 20 12:40:57 2008
