3s1s
From Proteopedia
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| - | [[ | + | ==Characterization and crystal structure of the type IIG restriction endonuclease BpuSI== |
| + | <StructureSection load='3s1s' size='340' side='right' caption='[[3s1s]], [[Resolution|resolution]] 2.35Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3s1s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_pumilus Bacillus pumilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S1S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S1S FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Bacillus pumilus ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1408 Bacillus pumilus])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s1s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s1s RCSB], [http://www.ebi.ac.uk/pdbsum/3s1s PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A type IIG restriction endonuclease, RM.BpuSI from Bacillus pumilus, has been characterized and its X-ray crystal structure determined at 2.35A resolution. The enzyme is comprised of an array of 5-folded domains that couple the enzyme's N-terminal endonuclease domain to its C-terminal target recognition and methylation activities. The REase domain contains a PD-x(15)-ExK motif, is closely superimposable against the FokI endonuclease domain, and coordinates a single metal ion. A helical bundle domain connects the endonuclease and methyltransferase (MTase) domains. The MTase domain is similar to the N6-adenine MTase M.TaqI, while the target recognition domain (TRD or specificity domain) resembles a truncated S subunit of Type I R-M system. A final structural domain, that may form additional DNA contacts, interrupts the TRD. DNA binding and cleavage must involve large movements of the endonuclease and TRD domains, that are probably tightly coordinated and coupled to target site methylation status. | ||
| - | + | Characterization and crystal structure of the type IIG restriction endonuclease RM.BpuSI.,Shen BW, Xu D, Chan SH, Zheng Y, Zhu Z, Xu SY, Stoddard BL Nucleic Acids Res. 2011 Jun 30. PMID:21724614<ref>PMID:21724614</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
*[[Endonuclease|Endonuclease]] | *[[Endonuclease|Endonuclease]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Bacillus pumilus]] | [[Category: Bacillus pumilus]] | ||
| - | [[Category: Chan, S H | + | [[Category: Chan, S H]] |
| - | [[Category: Shen, B W | + | [[Category: Shen, B W]] |
| - | [[Category: Stoddard, B L | + | [[Category: Stoddard, B L]] |
| - | [[Category: Xu, D | + | [[Category: Xu, D]] |
| - | [[Category: Xu, S Y | + | [[Category: Xu, S Y]] |
| - | [[Category: Zheng, Y | + | [[Category: Zheng, Y]] |
| - | [[Category: Zhu, Y | + | [[Category: Zhu, Y]] |
[[Category: Gamma-n6m-adenosine methyltransferase]] | [[Category: Gamma-n6m-adenosine methyltransferase]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: S-adenosyl-methionine binding]] | [[Category: S-adenosyl-methionine binding]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
Revision as of 11:00, 9 December 2014
Characterization and crystal structure of the type IIG restriction endonuclease BpuSI
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