1meh
From Proteopedia
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| - | [[Image:1meh.jpg|left|200px]] | + | [[Image:1meh.jpg|left|200px]] |
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| - | '''Inosine Monophosphate Dehydrogenase (IMPDH) From Tritrichomonas Foetus with IMP and MOA bound''' | + | {{Structure |
| + | |PDB= 1meh |SIZE=350|CAPTION= <scene name='initialview01'>1meh</scene>, resolution 1.95Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene> and <scene name='pdbligand=MOA:MYCOPHENOLIC ACID'>MOA</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/IMP_dehydrogenase IMP dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.205 1.1.1.205] | ||
| + | |GENE= IMPDH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5724 Tritrichomonas foetus]) | ||
| + | }} | ||
| + | |||
| + | '''Inosine Monophosphate Dehydrogenase (IMPDH) From Tritrichomonas Foetus with IMP and MOA bound''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MEH is a [ | + | 1MEH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Tritrichomonas_foetus Tritrichomonas foetus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MEH OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structures of Tritrichomonasfoetus inosine monophosphate dehydrogenase in complex with substrate, cofactor and analogs: a structural basis for the random-in ordered-out kinetic mechanism., Prosise GL, Luecke H, J Mol Biol. 2003 Feb 14;326(2):517-27. PMID:[http:// | + | Crystal structures of Tritrichomonasfoetus inosine monophosphate dehydrogenase in complex with substrate, cofactor and analogs: a structural basis for the random-in ordered-out kinetic mechanism., Prosise GL, Luecke H, J Mol Biol. 2003 Feb 14;326(2):517-27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12559919 12559919] |
[[Category: IMP dehydrogenase]] | [[Category: IMP dehydrogenase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: alpha beta barrel]] | [[Category: alpha beta barrel]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:42:34 2008'' |
Revision as of 10:42, 20 March 2008
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| , resolution 1.95Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Gene: | IMPDH (Tritrichomonas foetus) | ||||||
| Activity: | IMP dehydrogenase, with EC number 1.1.1.205 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Inosine Monophosphate Dehydrogenase (IMPDH) From Tritrichomonas Foetus with IMP and MOA bound
Overview
The enzyme inosine monophosphate dehydrogenase (IMPDH) is responsible for the rate-limiting step in guanine nucleotide biosynthesis. Because it is up-regulated in rapidly proliferating cells, human type II IMPDH is actively targeted for immunosuppressive, anticancer, and antiviral chemotherapy. The enzyme employs a random-in ordered-out kinetic mechanism where substrate or cofactor can bind first but product is only released after the cofactor leaves. Due to structural and kinetic differences between mammalian and microbial enzymes, most drugs that are successful in the inhibition of mammalian IMPDH are far less effective against the microbial forms of the enzyme. It is possible that with greater knowledge of the structural mechanism of the microbial enzymes, an effective and selective inhibitor of microbial IMPDH will be developed for use as a drug against multi-drug resistant bacteria and protists. The high-resolution crystal structures of four different complexes of IMPDH from the protozoan parasite Tritrichomonas foetus have been solved: with its substrate IMP, IMP and the inhibitor mycophenolic acid (MPA), the product XMP with MPA, and XMP with the cofactor NAD(+). In addition, a potassium ion has been located at the dimer interface. A structural model for the kinetic mechanism is proposed.
About this Structure
1MEH is a Single protein structure of sequence from Tritrichomonas foetus. Full crystallographic information is available from OCA.
Reference
Crystal structures of Tritrichomonasfoetus inosine monophosphate dehydrogenase in complex with substrate, cofactor and analogs: a structural basis for the random-in ordered-out kinetic mechanism., Prosise GL, Luecke H, J Mol Biol. 2003 Feb 14;326(2):517-27. PMID:12559919
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