1w4t
From Proteopedia
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Revision as of 12:37, 30 October 2007
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X-RAY CRYSTALLOGRAPHIC STRUCTURE OF PSEUDOMONAS AERUGINOSA ARYLAMINE N-ACETYLTRANSFERASE
Overview
The gene for NAT (arylamine N-acetyltransferase) from Pseudomonas, aeruginosa (panat) has been cloned from genomic DNA, and the gene product, (PANAT) expressed as an N-terminal histidine-tagged protein in Escherichia, coli and purified via nickel ion affinity chromatography. The specific, activities of PANAT against a broad range of substrates have been, investigated and compared with those of other prokaryotic NAT enzymes. For, most arylamine substrates identified, PANAT exhibits in vitro specific, activities typically one order of magnitude greater than those of, recombinant NAT enzymes from Mycobacterium smegmatis or Salmonella, typhimurium. Among the substrates of PANAT so far identified are the, anti-tubercular drug isoniazid, 5-aminosalicylate (a drug used in the, treatment of ... [(full description)]
About this Structure
1W4T is a [Single protein] structure of sequence from [[1]] with SO4 as [ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Expression, purification, characterization and structure of Pseudomonas aeruginosa arylamine N-acetyltransferase., Westwood IM, Holton SJ, Rodrigues-Lima F, Dupret JM, Bhakta S, Noble ME, Sim E, Biochem J. 2005 Jan 15;385(Pt 2):605-12. PMID:15447630
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