1mhx
From Proteopedia
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| - | [[Image:1mhx.jpg|left|200px]] | + | [[Image:1mhx.jpg|left|200px]] |
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| - | '''Crystal Structures of the redesigned protein G variant NuG1''' | + | {{Structure |
| + | |PDB= 1mhx |SIZE=350|CAPTION= <scene name='initialview01'>1mhx</scene>, resolution 1.8Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Crystal Structures of the redesigned protein G variant NuG1''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MHX is a [ | + | 1MHX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MHX OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structures and increased stabilization of the protein G variants with switched folding pathways NuG1 and NuG2., Nauli S, Kuhlman B, Le Trong I, Stenkamp RE, Teller D, Baker D, Protein Sci. 2002 Dec;11(12):2924-31. PMID:[http:// | + | Crystal structures and increased stabilization of the protein G variants with switched folding pathways NuG1 and NuG2., Nauli S, Kuhlman B, Le Trong I, Stenkamp RE, Teller D, Baker D, Protein Sci. 2002 Dec;11(12):2924-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12441390 12441390] |
[[Category: Finegoldia magna]] | [[Category: Finegoldia magna]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: redesigned first beta-hairpin]] | [[Category: redesigned first beta-hairpin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:43:34 2008'' |
Revision as of 10:43, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structures of the redesigned protein G variant NuG1
Overview
We recently described two protein G variants (NuG1 and NuG2) with redesigned first hairpins that were almost twice as stable, folded 100-fold faster, and had a switched folding mechanism relative to the wild-type protein. To test the structural accuracy of our design algorithm and to provide insights to the dramatic changes in the kinetics and thermodynamics of folding, we have now determined the crystal structures of NuG1 and NuG2 to 1.8 A and 1.85 A, respectively. We find that they adopt hairpin structures that are closer to the computational models than to wild-type protein G; the RMSD of the NuG1 hairpin to the design model and the wild-type structure are 1.7 A and 5.1 A, respectively. The crystallographic B factor in the redesigned first hairpin of NuG1 is systematically higher than the second hairpin, suggesting that the redesigned region is somewhat less rigid. A second round of structure-based design yielded new variants of NuG1 and NuG2, which are further stabilized by 0.5 kcal/mole and 0.9 kcal/mole.
About this Structure
1MHX is a Single protein structure of sequence from Finegoldia magna. Full crystallographic information is available from OCA.
Reference
Crystal structures and increased stabilization of the protein G variants with switched folding pathways NuG1 and NuG2., Nauli S, Kuhlman B, Le Trong I, Stenkamp RE, Teller D, Baker D, Protein Sci. 2002 Dec;11(12):2924-31. PMID:12441390
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