1mnv
From Proteopedia
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| - | [[Image:1mnv.gif|left|200px]] | + | [[Image:1mnv.gif|left|200px]] |
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| - | '''Actinomycin D binding to ATGCTGCAT''' | + | {{Structure |
| + | |PDB= 1mnv |SIZE=350|CAPTION= <scene name='initialview01'>1mnv</scene>, resolution 2.6Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Actinomycin D binding to ATGCTGCAT''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MNV is a [ | + | 1MNV is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MNV OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of actinomycin D bound to the CTG triplet repeat sequences linked to neurological diseases., Hou MH, Robinson H, Gao YG, Wang AH, Nucleic Acids Res. 2002 Nov 15;30(22):4910-7. PMID:[http:// | + | Crystal structure of actinomycin D bound to the CTG triplet repeat sequences linked to neurological diseases., Hou MH, Robinson H, Gao YG, Wang AH, Nucleic Acids Res. 2002 Nov 15;30(22):4910-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12433994 12433994] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Gao, Y G.]] | [[Category: Gao, Y G.]] | ||
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[[Category: Wang, A H.J.]] | [[Category: Wang, A H.J.]] | ||
[[Category: actinomycin d]] | [[Category: actinomycin d]] | ||
| - | [[Category: ctg triplet repeat | + | [[Category: ctg triplet repeat sequence]] |
[[Category: dna slippage]] | [[Category: dna slippage]] | ||
[[Category: mad]] | [[Category: mad]] | ||
| - | [[Category: neurological | + | [[Category: neurological disease]] |
[[Category: x-ray diffraction]] | [[Category: x-ray diffraction]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:45:49 2008'' |
Revision as of 10:45, 20 March 2008
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| , resolution 2.6Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Actinomycin D binding to ATGCTGCAT
Overview
The potent anticancer drug actinomycin D (ActD) acts by binding to DNA GpC sequences, thereby interfering with essential biological processes including replication, transcription and topoisomerase. Certain neurological diseases are correlated with expansion of (CTG)n trinucleotide sequences, which contain many contiguous GpC sites separated by a single base pair. In order to characterize the binding of ActD to CTG triplet repeat sequences, we carried out heat denaturation and CD analyses, which showed that adjacent GpC sequences flanking a T:T mismatch are preferred ActD-binding sites, and that ActD binding results in a conformational transition to A-type structure. The structural basis of the strong binding of ActD to neighboring GpC sites flanking a T:T mismatch was provided by the crystal structure of ActD bound to ATGCTGCAT, which contains a CTG triplet sequence. Binding of two ActD molecules to GCTGC causes a kink in the DNA helix. In addition, using a synthetic self-priming DNA model, 5'-(CAG)4(CTG)(16)-3', we observed that ActD can trap the cruciform or duplexes of (CTG)n and interfere with the expansion process of CTG triplet repeats as shown by gel electrophoretic expansion assay. Our results may provide the possible biological consequence of ActD bound to CTG triplet repeat sequences.
About this Structure
1MNV is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure of actinomycin D bound to the CTG triplet repeat sequences linked to neurological diseases., Hou MH, Robinson H, Gao YG, Wang AH, Nucleic Acids Res. 2002 Nov 15;30(22):4910-7. PMID:12433994
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