1mp1
From Proteopedia
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| - | [[Image:1mp1.gif|left|200px]] | + | [[Image:1mp1.gif|left|200px]] |
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| - | '''Solution structure of the PWI motif from SRm160''' | + | {{Structure |
| + | |PDB= 1mp1 |SIZE=350|CAPTION= <scene name='initialview01'>1mp1</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= SRm160 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Solution structure of the PWI motif from SRm160''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MP1 is a [ | + | 1MP1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MP1 OCA]. |
==Reference== | ==Reference== | ||
| - | Structure and function of the PWI motif: a novel nucleic acid-binding domain that facilitates pre-mRNA processing., Szymczyna BR, Bowman J, McCracken S, Pineda-Lucena A, Lu Y, Cox B, Lambermon M, Graveley BR, Arrowsmith CH, Blencowe BJ, Genes Dev. 2003 Feb 15;17(4):461-75. PMID:[http:// | + | Structure and function of the PWI motif: a novel nucleic acid-binding domain that facilitates pre-mRNA processing., Szymczyna BR, Bowman J, McCracken S, Pineda-Lucena A, Lu Y, Cox B, Lambermon M, Graveley BR, Arrowsmith CH, Blencowe BJ, Genes Dev. 2003 Feb 15;17(4):461-75. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12600940 12600940] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: four helix bundle]] | [[Category: four helix bundle]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:46:17 2008'' |
Revision as of 10:46, 20 March 2008
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| Gene: | SRm160 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of the PWI motif from SRm160
Overview
The PWI motif is a highly conserved domain of unknown function in the SRm160 splicing and 3'-end cleavage-stimulatory factor, as well as in several other known or putative pre-mRNA processing components. We show here that the PWI motif is a new type of RNA/DNA-binding domain that has an equal preference for single- and double-stranded nucleic acids. Deletion of the motif prevents SRm160 from binding RNA and stimulating 3'-end cleavage, and its substitution with a heterologous RNA-binding domain restores these functions. The NMR solution structure of the SRm160-PWI motif reveals a novel, four-helix bundle and represents the first example of an alpha-helical fold that can bind single-stranded (ss)RNA. Structure-guided mutagenesis indicates that the same surface is involved in RNA and DNA binding and requires the cooperative action of a highly conserved, adjacent basic region. Thus, the PWI motif is a novel type of nucleic acid-binding domain that likely has multiple important functions in pre-mRNA processing, including SRm160-dependent stimulation of 3'-end formation.
About this Structure
1MP1 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure and function of the PWI motif: a novel nucleic acid-binding domain that facilitates pre-mRNA processing., Szymczyna BR, Bowman J, McCracken S, Pineda-Lucena A, Lu Y, Cox B, Lambermon M, Graveley BR, Arrowsmith CH, Blencowe BJ, Genes Dev. 2003 Feb 15;17(4):461-75. PMID:12600940
Page seeded by OCA on Thu Mar 20 12:46:17 2008
