1mqv
From Proteopedia
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| - | [[Image:1mqv.gif|left|200px]] | + | [[Image:1mqv.gif|left|200px]] |
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| - | '''Crystal Structure of the Q1A/F32W/W72F mutant of Rhodopseudomonas palustris cytochrome c' (prime) expressed in E. coli''' | + | {{Structure |
| + | |PDB= 1mqv |SIZE=350|CAPTION= <scene name='initialview01'>1mqv</scene>, resolution 1.78Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of the Q1A/F32W/W72F mutant of Rhodopseudomonas palustris cytochrome c' (prime) expressed in E. coli''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MQV is a [ | + | 1MQV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhodopseudomonas_palustris Rhodopseudomonas palustris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MQV OCA]. |
==Reference== | ==Reference== | ||
| - | Structural features of cytochrome c' folding intermediates revealed by fluorescence energy-transfer kinetics., Lee JC, Engman KC, Tezcan FA, Gray HB, Winkler JR, Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14778-82. Epub 2002 Oct 29. PMID:[http:// | + | Structural features of cytochrome c' folding intermediates revealed by fluorescence energy-transfer kinetics., Lee JC, Engman KC, Tezcan FA, Gray HB, Winkler JR, Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14778-82. Epub 2002 Oct 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12407175 12407175] |
[[Category: Rhodopseudomonas palustris]] | [[Category: Rhodopseudomonas palustris]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: four-helix bundle]] | [[Category: four-helix bundle]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:47:00 2008'' |
Revision as of 10:47, 20 March 2008
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| , resolution 1.78Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Q1A/F32W/W72F mutant of Rhodopseudomonas palustris cytochrome c' (prime) expressed in E. coli
Overview
We employed fluorescence energy-transfer probes to investigate the polypeptide dynamics accompanying cytochrome c' folding. Analysis of fluorescence energy-transfer kinetics from wild-type Trp-72 or Trp-32 in a crystallographically characterized (1.78 A) Q1A/F32W/W72F mutant shows that there is structural heterogeneity in denatured cytochrome c'. Even at guanidine hydrochloride concentrations well beyond the unfolding transition, a substantial fraction of the polypeptides ( approximately 50%) adopts compact conformations (tryptophan-to-heme distance, approximately 25 A) in both pseudo-wild-type (Q1A) and mutant proteins. A burst phase (< or =5 ms) is revealed when stopped flow-triggered refolding is probed by tryptophan intensity: measurements on the Q1A protein show that approximately 75% of the Trp-72 fluorescence (83% for Trp-32) is quenched within the mixing deadtime, suggesting that most of the polypeptides have collapsed.
About this Structure
1MQV is a Single protein structure of sequence from Rhodopseudomonas palustris. Full crystallographic information is available from OCA.
Reference
Structural features of cytochrome c' folding intermediates revealed by fluorescence energy-transfer kinetics., Lee JC, Engman KC, Tezcan FA, Gray HB, Winkler JR, Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14778-82. Epub 2002 Oct 29. PMID:12407175
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