1mvm
From Proteopedia
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| - | [[Image:1mvm.gif|left|200px]] | + | [[Image:1mvm.gif|left|200px]] |
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| - | '''MVM(STRAIN I), COMPLEX(VIRAL COAT/DNA), VP2, PH=7.5, T=4 DEGREES C''' | + | {{Structure |
| + | |PDB= 1mvm |SIZE=350|CAPTION= <scene name='initialview01'>1mvm</scene>, resolution 3.500Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''MVM(STRAIN I), COMPLEX(VIRAL COAT/DNA), VP2, PH=7.5, T=4 DEGREES C''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MVM is a [ | + | 1MVM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Minute_virus_of_mice Minute virus of mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVM OCA]. |
==Reference== | ==Reference== | ||
| - | Structure determination of minute virus of mice., Llamas-Saiz AL, Agbandje-McKenna M, Wikoff WR, Bratton J, Tattersall P, Rossmann MG, Acta Crystallogr D Biol Crystallogr. 1997 Jan 1;53(Pt 1):93-102. PMID:[http:// | + | Structure determination of minute virus of mice., Llamas-Saiz AL, Agbandje-McKenna M, Wikoff WR, Bratton J, Tattersall P, Rossmann MG, Acta Crystallogr D Biol Crystallogr. 1997 Jan 1;53(Pt 1):93-102. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15299974 15299974] |
[[Category: Minute virus of mice]] | [[Category: Minute virus of mice]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: viral coat protein/nucleic acid]] | [[Category: viral coat protein/nucleic acid]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:48:45 2008'' |
Revision as of 10:48, 20 March 2008
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| , resolution 3.500Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
MVM(STRAIN I), COMPLEX(VIRAL COAT/DNA), VP2, PH=7.5, T=4 DEGREES C
Overview
The three-dimensional crystal structure of the single-stranded DNA-containing ('full') parvovirus, minute virus of mice (MVM), has been determined to 3.5 A resolution. Both full and empty particles of MVM were crystallized in the monoclinic space group C2 with cell dimensions of a = 448.7, b = 416.7, c = 305.3 A and beta = 95.8 degrees. Diffraction data were collected at the Cornell High Energy Synchrotron Source using an oscillation camera. The crystals have a pseudo higher R32 space group in which the particles are situated at two special positions with 32 point symmetry, separated by (1/2)c in the hexagonal setting. The self-rotation function showed that the particles are rotated with respect to each other by 60 degrees around the pseudo threefold axis. Subsequently, a more detailed analysis of the structure amplitudes demonstrated that the correct space-group symmetry is C2 as given above. Only one of the three twofold axes perpendicular to the threefold axis in the pseudo R32 space group is a 'true' crystallographic twofold axis; the other two are only 'local' non-crystallographic symmetry axes. The known canine parvovirus (CPV) structure was used as a phasing model to initiate real-space electron-density averaging phase improvement. The electron density was easily interpretable and clearly showed the amino-acid differences between MVM and CPV, although the final overall correlation coefficient was only 0.63. The structure of MVM has a large amount of icosahedrally ordered DNA, amounting to 22% of the viral genome, which is significantly more than that found in CPV.
About this Structure
1MVM is a Single protein structure of sequence from Minute virus of mice. Full crystallographic information is available from OCA.
Reference
Structure determination of minute virus of mice., Llamas-Saiz AL, Agbandje-McKenna M, Wikoff WR, Bratton J, Tattersall P, Rossmann MG, Acta Crystallogr D Biol Crystallogr. 1997 Jan 1;53(Pt 1):93-102. PMID:15299974
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