1mw4
From Proteopedia
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| - | [[Image:1mw4.jpg|left|200px]] | + | [[Image:1mw4.jpg|left|200px]] |
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| - | '''Solution structure of the human Grb7-SH2 domain in complex with a 10 amino acid peptide pY1139''' | + | {{Structure |
| + | |PDB= 1mw4 |SIZE=350|CAPTION= <scene name='initialview01'>1mw4</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Solution structure of the human Grb7-SH2 domain in complex with a 10 amino acid peptide pY1139''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MW4 is a [ | + | 1MW4 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MW4 OCA]. |
==Reference== | ==Reference== | ||
| - | Solution structure of the human Grb7-SH2 domain/erbB2 peptide complex and structural basis for Grb7 binding to ErbB2., Ivancic M, Daly RJ, Lyons BA, J Biomol NMR. 2003 Nov;27(3):205-19. PMID:[http:// | + | Solution structure of the human Grb7-SH2 domain/erbB2 peptide complex and structural basis for Grb7 binding to ErbB2., Ivancic M, Daly RJ, Lyons BA, J Biomol NMR. 2003 Nov;27(3):205-19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12975581 12975581] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: sh2 domain in complex with a ligand]] | [[Category: sh2 domain in complex with a ligand]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:48:56 2008'' |
Revision as of 10:48, 20 March 2008
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| Activity: | Transferase, with EC number and 2.7.10.2 2.7.10.1 and 2.7.10.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of the human Grb7-SH2 domain in complex with a 10 amino acid peptide pY1139
Contents |
Overview
The solution structure of the hGrb7-SH2 domain in complex with a ten amino acid phosphorylated peptide ligand representative of the erbB2 receptor tyrosine kinase (pY1139) is presented as determined by nuclear magnetic resonance methods. The hGrb7-SH2 domain structure reveals the Src homology 2 domain topology consisting of a central beta-sheet capped at each end by an alpha-helix. The presence of a four residue insertion in the region between beta-strand E and the EF loop and resulting influences on the SH2 domain/peptide complex structure are discussed. The binding conformation of the erbB2 peptide is in a beta-turn similar to that found in phosphorylated tyrosine peptides bound to the Grb2-SH2 domain. To our knowledge this is only the second example of an SH2 domain binding its naturally occurring ligands in a turn, instead of extended, conformation. Close contacts between residues responsible for binding specificity in hGrb7-SH2 and the erbB2 peptide are characterized and the potential effect of mutation of these residues on the hGrb7-SH2 domain structure is discussed.
Disease
Known diseases associated with this structure: Adenocarcinoma of lung, somatic OMIM:[164870], Gastric cancer, somatic OMIM:[164870], Glioblastoma, somatic OMIM:[164870], Ovarian cancer, somatic, OMIM:[164870], Sialidosis, type I OMIM:[608272], Sialidosis, type II OMIM:[608272]
About this Structure
1MW4 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Solution structure of the human Grb7-SH2 domain/erbB2 peptide complex and structural basis for Grb7 binding to ErbB2., Ivancic M, Daly RJ, Lyons BA, J Biomol NMR. 2003 Nov;27(3):205-19. PMID:12975581
Page seeded by OCA on Thu Mar 20 12:48:56 2008
