1mwe
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1mwe.gif|left|200px]] | + | [[Image:1mwe.gif|left|200px]] |
| - | + | ||
| - | '''THE X-RAY STRUCTURE OF A COMPLEX OF TERN N9 INFLUENZA VIRUS NEURAMINIDASE COMPLEXED WITH SIALIC ACID AT 4 DEGREES C REVEALING A SECOND SIALIC ACID BINDING SITE''' | + | {{Structure |
| + | |PDB= 1mwe |SIZE=350|CAPTION= <scene name='initialview01'>1mwe</scene>, resolution 1.7Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene> and <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''THE X-RAY STRUCTURE OF A COMPLEX OF TERN N9 INFLUENZA VIRUS NEURAMINIDASE COMPLEXED WITH SIALIC ACID AT 4 DEGREES C REVEALING A SECOND SIALIC ACID BINDING SITE''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1MWE is a [ | + | 1MWE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWE OCA]. |
==Reference== | ==Reference== | ||
| - | Structural evidence for a second sialic acid binding site in avian influenza virus neuraminidases., Varghese JN, Colman PM, van Donkelaar A, Blick TJ, Sahasrabudhe A, McKimm-Breschkin JL, Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):11808-12. PMID:[http:// | + | Structural evidence for a second sialic acid binding site in avian influenza virus neuraminidases., Varghese JN, Colman PM, van Donkelaar A, Blick TJ, Sahasrabudhe A, McKimm-Breschkin JL, Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):11808-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9342319 9342319] |
[[Category: Exo-alpha-sialidase]] | [[Category: Exo-alpha-sialidase]] | ||
[[Category: Influenza a virus]] | [[Category: Influenza a virus]] | ||
| Line 25: | Line 34: | ||
[[Category: sialic acid binding]] | [[Category: sialic acid binding]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:49:03 2008'' |
Revision as of 10:49, 20 March 2008
| |||||||
| , resolution 1.7Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Activity: | Exo-alpha-sialidase, with EC number 3.2.1.18 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE X-RAY STRUCTURE OF A COMPLEX OF TERN N9 INFLUENZA VIRUS NEURAMINIDASE COMPLEXED WITH SIALIC ACID AT 4 DEGREES C REVEALING A SECOND SIALIC ACID BINDING SITE
Overview
The x-ray structure of a complex of sialic acid (Neu5Ac) with neuraminidase N9 subtype from A/tern/Australia/G70C/75 influenza virus at 4 degrees C has revealed the location of a second Neu5Ac binding site on the surface of the enzyme. At 18 degrees C, only the enzyme active site contains bound Neu5Ac. Neu5Ac binds in the second site in the chair conformation in a similar way to which it binds to hemagglutinin. The residues that interact with Neu5Ac at this second site are mostly conserved in avian strains, but not in human and swine strains, indicating that it has some as-yet-unknown biological function in birds.
About this Structure
1MWE is a Single protein structure of sequence from Influenza a virus. Full crystallographic information is available from OCA.
Reference
Structural evidence for a second sialic acid binding site in avian influenza virus neuraminidases., Varghese JN, Colman PM, van Donkelaar A, Blick TJ, Sahasrabudhe A, McKimm-Breschkin JL, Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):11808-12. PMID:9342319
Page seeded by OCA on Thu Mar 20 12:49:03 2008
