2itg

Publication Abstract from PubMed

We solved the structure and traced the complete active site of the catalytic domain of the human immunodeficiency virus type 1 integrase (HIV-1 IN) with the F185H mutation. The only previously available crystal structure, the F185K mutant of this domain, lacks one of the catalytically important residues, E152, located in a stretch of 12 disordered residues [Dyda et al. (1994) Science 266, 1981-1986]. It is clear, however, that the active site of HIV-1 IN observed in either structure cannot correspond to that of the functional enzyme, since the cluster of three conserved carboxylic acids does not create a proper metal-binding site. The conformation of the loop was compared with two different conformations found in the catalytic domain of the related avian sarcoma virus integrase [Bujacz et al. (1995) J. Mol. Biol. 253, 333-346]. Flexibility of the active site region of integrases may be required in order for the enzyme to assume a functional conformation in the presence of substrate and/or cofactors.

The catalytic domain of human immunodeficiency virus integrase: ordered active site in the F185H mutant.,Bujacz G, Alexandratos J, Qing ZL, Clement-Mella C, Wlodawer A FEBS Lett. 1996 Dec 2;398(2-3):175-8. PMID:8977101^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.