1mxs
From Proteopedia
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| - | [[Image:1mxs.gif|left|200px]] | + | [[Image:1mxs.gif|left|200px]] |
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| - | '''Crystal structure of 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase from Pseudomonas putida.''' | + | {{Structure |
| + | |PDB= 1mxs |SIZE=350|CAPTION= <scene name='initialview01'>1mxs</scene>, resolution 2.20Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/2-dehydro-3-deoxy-phosphogluconate_aldolase 2-dehydro-3-deoxy-phosphogluconate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.14 4.1.2.14] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase from Pseudomonas putida.''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MXS is a [ | + | 1MXS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MXS OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase from Pseudomonas putida., Bell BJ, Watanabe L, Rios-Steiner JL, Tulinsky A, Lebioda L, Arni RK, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1454-8. Epub 2003, Jul 23. PMID:[http:// | + | Structure of 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase from Pseudomonas putida., Bell BJ, Watanabe L, Rios-Steiner JL, Tulinsky A, Lebioda L, Arni RK, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1454-8. Epub 2003, Jul 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12876349 12876349] |
[[Category: 2-dehydro-3-deoxy-phosphogluconate aldolase]] | [[Category: 2-dehydro-3-deoxy-phosphogluconate aldolase]] | ||
[[Category: Pseudomonas putida]] | [[Category: Pseudomonas putida]] | ||
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[[Category: sulfate]] | [[Category: sulfate]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:49:34 2008'' |
Revision as of 10:49, 20 March 2008
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| , resolution 2.20Å | |||||||
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| Ligands: | |||||||
| Activity: | 2-dehydro-3-deoxy-phosphogluconate aldolase, with EC number 4.1.2.14 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase from Pseudomonas putida.
Overview
2-Keto-3-deoxy-6-phosphogluconate (KDPG) aldolase from Pseudomonas putida is a key enzyme in the Entner-Doudoroff pathway which catalyses the cleavage of KDPG via a class I Schiff-base mechanism. The crystal structure of this enzyme has been refined to a crystallographic residual R = 17.1% (R(free) = 21.4%). The N-terminal helix caps one side of the torus of the (betaalpha)(8)-barrel and the active site is located on the opposite, carboxylic side of the barrel. The Schiff-base-forming Lys145 is coordinated by a sulfate (or phosphate) ion and two solvent water molecules. The interactions that stabilize the trimer are predominantly hydrophobic, with the exception of the cyclically permuted bonds formed between Glu132 OE1 of one molecule and Thr129 OG1 of a symmetry-equivalent molecule. Except for the N-terminal helix, the structure of KDPG aldolase from P. putida closely resembles the structure of the homologous enzyme from Escherichia coli.
About this Structure
1MXS is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.
Reference
Structure of 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase from Pseudomonas putida., Bell BJ, Watanabe L, Rios-Steiner JL, Tulinsky A, Lebioda L, Arni RK, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1454-8. Epub 2003, Jul 23. PMID:12876349
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