1mzm

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[[Image:1mzm.jpg|left|200px]]<br /><applet load="1mzm" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1mzm.jpg|left|200px]]
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caption="1mzm, resolution 1.78&Aring;" />
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'''MAIZE NONSPECIFIC LIPID TRANSFER PROTEIN COMPLEXED WITH PALMITATE'''<br />
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{{Structure
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|PDB= 1mzm |SIZE=350|CAPTION= <scene name='initialview01'>1mzm</scene>, resolution 1.78&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene> and <scene name='pdbligand=FMT:FORMIC ACID'>FMT</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''MAIZE NONSPECIFIC LIPID TRANSFER PROTEIN COMPLEXED WITH PALMITATE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1MZM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays] with <scene name='pdbligand=PLM:'>PLM</scene> and <scene name='pdbligand=FMT:'>FMT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZM OCA].
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1MZM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZM OCA].
==Reference==
==Reference==
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High-resolution crystal structure of the non-specific lipid-transfer protein from maize seedlings., Shin DH, Lee JY, Hwang KY, Kim KK, Suh SW, Structure. 1995 Feb 15;3(2):189-99. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7735835 7735835]
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High-resolution crystal structure of the non-specific lipid-transfer protein from maize seedlings., Shin DH, Lee JY, Hwang KY, Kim KK, Suh SW, Structure. 1995 Feb 15;3(2):189-99. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7735835 7735835]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Zea mays]]
[[Category: Zea mays]]
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[[Category: lipid transport]]
[[Category: lipid transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:00:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:50:11 2008''

Revision as of 10:50, 20 March 2008

Image:1mzm.jpg


PDB ID 1mzm

Drag the structure with the mouse to rotate
, resolution 1.78Å
Ligands: and
Coordinates: save as pdb, mmCIF, xml



MAIZE NONSPECIFIC LIPID TRANSFER PROTEIN COMPLEXED WITH PALMITATE


Overview

BACKGROUND: The movement of lipids between membranes is aided by lipid-transfer proteins (LTPs). Some LTPs exhibit broad specificity, transferring many classes of lipids, and are termed non-specific LTPs (ns-LTPs). Despite their apparently similar mode of action, no sequence homology exists between mammalian and plant ns-LTPs and no three-dimensional structure has been reported for any plant ns-LTP. RESULTS: We have determined the crystal structure of ns-LTP from maize seedlings by multiple isomorphous replacement and refined the structure to 1.9 A resolution. The protein comprises a single compact domain with four alpha-helices and a long C-terminal region. The eight conserved cysteines form four disulfide bridges (assigned as Cys4-Cys52, Cys14-Cys29, Cys30-Cys75, and Cys50-Cys89) resolving the ambiguity that remained from the chemical determination of pairings in the homologous protein from castor bean. Two of the bonds, Cys4-Cys52 and Cys50-Cys89, differ from what would have been predicted from sequence alignment with soybean hydrophobic protein. The complex between maize ns-LTP and hexadecanoate (palmitate) has also been crystallized and its structure refined to 1.8 A resolution. CONCLUSIONS: The fold of maize ns-LTP places it in a new category of all-alpha-type structure, first described for soybean hydrophobic protein. In the absence of a bound ligand, the protein has a tunnel-like hydrophobic cavity, which is large enough to accommodate a long fatty acyl chain. In the structure of the complex with palmitate, most of the acyl chain is buried inside this hydrophobic cavity.

About this Structure

1MZM is a Single protein structure of sequence from Zea mays. Full crystallographic information is available from OCA.

Reference

High-resolution crystal structure of the non-specific lipid-transfer protein from maize seedlings., Shin DH, Lee JY, Hwang KY, Kim KK, Suh SW, Structure. 1995 Feb 15;3(2):189-99. PMID:7735835

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